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Isolation and biological and genotypic characterization of Toxoplasma gondii from wild animals from Brazil

Grant number: 09/00175-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2009
Effective date (End): April 30, 2012
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Solange Maria Gennari
Grantee:Sérgio Netto Vitaliano
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Toxoplasma gondii is an intracellular protozoan parasite which is capable to infect almost all warm-blooded animals, including humans. Although research indicates that wild animals are frequently positives for T. gondii, the role of wild life in this parasite epidemiology is not well understood, neither which genotypic profile is predominant in infected wild animals. Genotypic characterization, based on PCR-RFLP markers, of different isolates has been realized mostly with samples from humans, sheep, goats, dogs, cats, and birds, and less frequently with wild animals. Experimental data, mainly from the analysis of the locus SAG2, obtained from humans and domestic animals from Europe and North America indicates that T. gondii has a clonal population structure with three distinct genetic lineages (Types I, II, III). However, this affirmative is not valid in South America and with wild animals. Recent studies revealed higher genetic diversity in South America and with wild animals in general, including the discovery of new genotypes. The aim of this study is to isolate and characterize genetically T. gondii obtained from free ranging and captive wild birds and mammals from different localizations from Brazil.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PENA, H. F. J.; MARVULO, M. F. V.; HORTA, M. C.; SILVA, M. A.; SILVA, J. C. R.; SIQUEIRA, D. B.; LIMA, P. -A. C. P.; VITALIANO, S. N.; GENNARI, S. M. Isolation and genetic characterisation of Toxoplasma gondii from a red-handed howler monkey (Alouatta belzebul), a jaguarundi (Puma yagouaroundi), and a black-eared opossum (Didelphis aurita) from Brazil. Veterinary Parasitology, v. 175, n. 3-4, p. 377-381, FEB 10 2011. Web of Science Citations: 34.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VITALIANO, Sérgio Netto. Isolamento e caracterização biológica e genotípica de Toxoplasma gondii em animais selvagens do Brasil. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.