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The effect of Yerba Maté aqueous extract (Ilex paraguariensis) ingestion on nuclear factor kappa B signaling pathway in peritoneal macrophages of rats fed a high-fat diet

Grant number: 09/02371-0
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2009
Effective date (End): August 31, 2011
Field of knowledge:Health Sciences - Nutrition
Principal researcher:Marcelo Macedo Rogero
Grantee:Maria Carolina Borges
Home Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Obesity-induced chronic inflammation is associated with macrophages migration to adipose tissue, which produce proinflammatory cytokines, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)- 1 e IL-6, contributing to type 2 diabetes mellitus and metabolic syndrome pathogenesis. In macrophages, the modulation of gene expression of many cytokines involved in inflammatory processes is controlled by a transcription factor named nuclear factor-kappa B (NF-kappa B). Yerba Maté (Ilex paraguariensis) contains bioactive compounds, such as cafeic acid, kaempferol and quercetin, which reduce in vitro NF-kappaB activation in macrophages. This project aims to investigate the effect of Yerba Maté aqueous extract ingestion on NF-kappaB signaling pathway in peritoneal macrophages of rats fed a high-fat diet. Wistar male rats will be fed a control diet (n= 30) or a high-fat diet (n= 30) during 12 weeks. After this period, 10 animals from each group will be sacrificed, while the remaining animals will be distributed to two groups: one of which will receive, by gavage, Yerba Maté aqueous extract (1 g/kg body weight) for four weeks. Then, the peritoneal macrophages will be collected, stimulated with lipopolysaccharides (LPS) and used to evaluate TNF-alpha, IL-1beta e IL-6 mRNAs expression; NF-kappaB activation; IKK-beta and IKB-alpha (total and phosphorylated) expression and in vitro synthesis of TNF-alpha, IL-1beta e IL-6. Body composition of animals' carcasses will be analyzed and the blood will be used for the determination of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, triacilglicerols, insulin, leptin, adiponectin, TNF-alpha, IL-6, monocyte chemotactic protein (MCP)-1, plasminogen activator inhibitor (PAI)-1, alanin aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORGES, MARIA CAROLINA; RAMIREZ VINOLO, MARCO AURELIO; NAKAJIMA, KARINA; DE CASTRO, INAR ALVESDE; MARKOWICZ BASTOS, DEBORAH HELENA; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO; TIRAPEGUI, JULIO; CURI, RUI; ROGERO, MARCELO MACEDO. The effect of mate tea (Ilex paraguariensis) on metabolic and inflammatory parameters in high-fat diet-fed Wistar rats. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, v. 64, n. 5, p. 561-569, AUG 2013. Web of Science Citations: 14.
BORGES, MARIA C.; VINOLO, MARCO A. R.; CRISMA, AMANDA R.; FOCK, RICARDO A.; BORELLI, PRIMAVERA; TIRAPEGUI, JULIO; CURI, RUI; ROGERO, MARCELO M. High-fat diet blunts activation of the nuclear factor-kappa B signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats. NUTRITION, v. 29, n. 2, p. 443-449, FEB 2013. Web of Science Citations: 11.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.