Metabolomic analysis in tumor cellular lineages treated with conjugated linoleic acid by Nuclear Magnetic Resonance

• Grant number: 09/17846-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2010
• End date: November 30, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Luiz Alberto Colnago
• Grantee: Roberta Manzano Maria
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil

Abstract

NMR is one of the most used techniques to analyze chemical and biological samples. It is one of the main tools to analyze metabolic profile (metabolomics) both in vitro and in vivo. In this project, NMR spectroscopy in high resolution will be used to the qualitative and quantitative analysis of breast cancer cellular lineages MDA-MB-231 and MCF-7 and prostate cancer cells DU-145, treated with conjugated linoleic acid. CLA, a fatty acid found in ruminant animals meat, has benefic potential effects in human health, among them a possible action against some cancer types. Tumor cells will be also analyzed with spectroscopy in infrared zone, with Fourier Transform.Metabolic changes among health, tumor and CLA treated cells will be analyzed to verify possible effects caused by this fatty acid. Besides this research, CLA influences in angiogenic activity as well as changes occurred during the apoptosis process is intended to be analyzed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARIA, ROBERTA M.; ALTEI, WANESSA F.; VALADARES, NAPOLEAO F.; GARRATT, RICHARD C.; ANDRICOPULO, ADRIANO D.; VENANCIO, TIAGO; COLNAGO, LUIZ A.. Effect of cis-9, trans-11 Conjugated Linoleic Acid (CLA) on the Metabolism Profile of Breast Cancer Cells Determined by H-1 HR-MAS NMR Spectroscopy. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 3-7, JAN 2019. (13/05128-5, 09/17846-4)

ROBERTA M. MARIA; WANESSA F. ALTEI; NAPOLEÃO F. VALADARES; RICHARD C. GARRATT; ADRIANO D. ANDRICOPULO; TIAGO VENÂNCIO; LUIZ A. COLNAGO. Effect of cis-9, trans-11 Conjugated Linoleic Acid (CLA) on the Metabolism Profile of Breast Cancer Cells Determined by H HR-MAS NMR Spectroscopy. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 3-7, 2019-01-00. (13/05128-5, 09/17846-4)

MARIA, ROBERTA M.; ALTEI, WANESSA F.; ANDRICOPULO, ADRIANO D.; BECCENERI, AMANDA B.; COMINETTI, MARCIA R.; VENANCIO, TIAGO; COLNAGO, LUIZ A.. Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy. Analytical Biochemistry, v. 488, p. 14-18, NOV 1 2015. (09/17846-4, 13/05128-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARIA, Roberta Manzano. Metabolism study of breast cancer cells subjected to CLA using NMR. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.