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Quality of the gametes and artificial fertilization of surubim-do-Paraíba, Steindachneridion parahybae

Grant number: 09/18609-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2010
Effective date (End): October 31, 2012
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Elizabeth Romagosa
Grantee:Eduardo Antonio Sanches
Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


The surubim-Paraíba (Steindachneridion parahybae) is a migratory fish river basin of the river Paraíba-do-sul, and currently is in the lists of endangered fish. Great concern about the conservation of genetic biodiversity and reforestation programs with this species has been raised, however, little is known about the reproductive management in captivity, adopting this practice for some empirical measures. The research objective was to generate basic information on the artificial breeding of surubim-Paraíba, especially with regard to the quality of gametes, artificial fertilization management and short-term storage of gametes. The experiment will be conducted in Hydrobiology STATION AND AQUACULTURE COMPANY ENERGY OF SAO PAULO (EHA/CESP), Paraibuna, Sao Paulo, Brazil. It Will be conducted two experimental studies in experimental factorial: (1) To evaluate the optimal sperm / oocytes / water used in artificial fertilization to dry, for both, the treatment will consist of five insemination doses (5,000, 50,000, 500,000, 5,000,000 and 50,000,000 spermatozoon.ovócitos-1 ) three volumes of water (10, 35 and 60 mL of water.mL-1 oocyte) and three replicas, (2) evaluate the effect of storage temperature on the short-gametes-out will be divided into two phases (2.1.) storage of oocytes in four exposure times (0, 60, 120 and 180 min. post-collection) at four temperatures of exposure (5, 15, 25 and 35 C) in three replicas (2.2) storage Semen assessments on eight times (0, 2, 4, 8, 16, 32, 48 and 64 hours post-collection) at four different temperatures (5, 15, 25 and 35 degrees Celsius) with 10 replicates (10 males). The results of these tests may serve as a basis for future programs of reforestation, conservation of genetic biodiversity, and, providing the optimization of arrays through the rational use of their gametes

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANCHES, EDUARDO ANTONIO; CANEPPELE, DANILO; OKAWARA, RENAN YOSHIHARU; DAMASCENO, DANIELLE ZANERATO; BOMBARDELLI, ROBIE ALLAN; ROMAGOSA, ELIZABETH. Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish. Neotropical Ichthyology, v. 14, n. 1 2016. Web of Science Citations: 2.
SANCHES, E. A.; OKAWARA, R. Y.; CANEPPELE, D.; TOLEDO, C. P. R.; BOMBARDELLI, R. A.; ROMAGOSA, E. Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112h of storage at four temperatures. Journal of Applied Ichthyology, v. 31, n. 1, SI, p. 79-88, JUN 2015. Web of Science Citations: 5.
SANCHES, EDUARDO ANTONIO; OKAWARA, RENAN YOSHIHARU; CANEPPELE, DANILO; NEUMANN, GIOVANO; BOMBARDELLI, ROBIE ALLAN; ROMAGOSA, ELIZABETH. Storage of Steindachneridion parahybae oocytes at different temperatures. Animal Reproduction Science, v. 151, n. 3-4, p. 262-268, DEC 30 2014. Web of Science Citations: 7.
SANCHES, E. A.; MARCOS, R. M.; OKAWARA, R. Y.; CANEPPELE, D.; BOMBARDELLI, R. A.; ROMAGOSA, E. Sperm motility parameters for Steindachneridion parahybae based on open-source software. Journal of Applied Ichthyology, v. 29, n. 5, p. 1114-1122, OCT 2013. Web of Science Citations: 13.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANCHES, Eduardo Antonio. Metodologias de avaliação de sêmen e procedimentos de fertilização artificial de surubim-do-paraíba, Steindachneridion parahybae. 2012. 137 f. Doctoral Thesis - Universidade Estadual Paulista Centro de Aquicultura.

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