miRNA signature of T lymphocyte subpopulations from healthy and multiple sclerosis patients

• Grant number: 10/00310-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2010
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Wilson Araújo da Silva Junior
• Grantee: Julio Cesar Cetrulo Lorenzi
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 12/15746-5 - Evaluation of epigenetic status of miRNA markers for multiple sclerosis, BE.EP.DR

Abstract

The pathology of multiple sclerosis (MS) is typically characterized by perivenular lymphocyte infiltration, destruction of the myelin sheath and axonal loss. The myelin sheath proteins are considered the main targets for T lymphocyte attack. Evidence on the induction and perpetuation of MS still favors CD4+ autoreactive T lymphocytes as a central factor for the autoimmune pathogenesis of MS, in the same way the Regulatory T lymphocytes have emerged as crucial player in the MS. The study of gene expression of different T lymphocytes subsets involved in this disease can determine the possible mechanisms involved in its pathogenesis. Among the different classes of molecules capable of regulating gene expression microRNAs (miRNAs) have aroused great interest in autoimmune diseases. The miRNAs are small RNAs encoded in the human genome that has control function protein expression from the degradation of messenger RNA. Several miRNAs have been described with the regulation of the maturation and differentiation of T lymphocytes and thereby possibly they may be deregulated in MS. Thus the objective of this project is to verify if there is gene expression signature of miRNA in lymphocytes and subsets in healthy patients and with MS in order to better understand the molecular mechanism of this disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CETRULO LORENZI, JULIO CESAR; BRUM, DORALINA G.; ZANETTE, DALILA L.; ALVES SOUZA, ALESSANDRA DE PAULA; BARBUZANO, FERNANDA GONCALVES; DOS SANTOS, ANTONIO CARLOS; BARREIRA, AMILTON ANTUNES; SILVA, JR., WILSON ARAUJO. miR-15a and 16-1 Are Downregulated in CD4(+) T Cells of Multiple Sclerosis Relapsing Patients. International Journal of Neuroscience, v. 122, n. 8, p. 466-471, AUG 2012. (98/14247-6, 10/00310-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LORENZI, Julio Cesar Cetrulo. miRNA expression analysis in T lymphocytes subpopulations from multiple sclerosis patients. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.