| Grant number: | 10/07133-8 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Start date: | October 01, 2010 |
| End date: | September 30, 2013 |
| Field of knowledge: | Biological Sciences - Biochemistry - Molecular Biology |
| Principal Investigator: | Richard John Ward |
| Grantee: | Lucas Ferreira Ribeiro |
| Host Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated research grant: | 08/57908-6 - National Institute of Science and Technology of Bioethanol, AP.BIOEN.TEM |
Abstract The project proposes the use of a new approach for directed evolution. It allows the creation of allosteric proteins by In Vitro Recombination of Nonhomologous Genes. This technique allows to "evolve" enzymes so it can be stimulated by the product, without the need to change the catalytic site, which could lead to a decrease in enzymatic activity. Using a library of random domain insertion, which had its variability increased by random circular permutation of one domain, we can then select chimeric mutant proteins with allosteric properties. It will be adopted as a model for studying an enzyme with industrial applications and feature mesophilic (Bacillus subtilis endoxylanase A). This will randomly link a periplasmic binding protein to D-xylose (XBP), aiming to create a domain interface, which could lead to a positive modulation on xylanase activity, due to the binding of D-xylose. This strategy will provide a more detailed study of molecular mapping which will provide an interesting platform to explore the structural basis on how to evolve the allosteric in nature. | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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