Identification and validation of molecular markers for the risk of breast ductal carcinoma progression

• Grant number: 09/00669-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2009
• End date: July 31, 2012
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Dirce Maria Carraro
• Grantee: Carolina Sens Abuázar
• Host Institution: Hospital A C Camargo. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil

Abstract

Ductal carcinoma in situ (DCIS) represents the most common kind of breast cancer. DCIS constitutes a heterogeneous group of tumors which can rapidily evolve to the invasive form of the disease or change slowly after a long period of the disease. Our research group has been working in the characterization of a genetic expression pattern of the metastasis process through the combination of two methodologies: microarray and laser microdissection.In a previous study (Castro, 2008) the concept of molecular divergence was applied in independent groups of samples, which represent the breast cancer progression [Normal epithelium, pure DCIS, the in situ component of the lesion that coexists with the invasive ductal carcinoma (DCIS-IDC)and the invasive ductal carcinoma (IDC). The results showed that the pattern of expression of the in situ component of DCIS-IDC is similar to IDC cells but differ molecularly from the cells of the pure in situ component (DCIS). These results suggest that the molecular modifications of the in situ component of DCIS-IDC cells occur before the morphological alterations take place,indicating a group of genes with the potentiality to predict the risk of pure DCIS progression (Castro et al., 2008). In the sequence, based on our previos results, we intend to evaluate these data through Real Time PCR and immunohistochemistry, using a tissue microarray (TMA), containing samples of pure DCIS and the in situ component of DCIS-IDC. Moreover, this study intends to investigate the genetic expression changes of the myoepithelial cells in pure DCIS and in the in situ component of DCIS-IDC, once these cells seem to present a determined role in the progression of breast ductal carcinoma (Hu et al., 2008).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SENS-ABUAZAR, CAROLINA; NAPOLITANO E FERREIRA, ELISA; BUENO TOLEDO OSORIO, CYNTHIA APARECIDA; VICTORINO KREPISCHI, ANA CRISTINA; RICCA, TATIANA IERVOLINO; CASTRO, NADIA PEREIRA; DA CUNHA, ISABELA WERNECK; MACIEL, MARIA DO SOCORRO; ROSENBERG, CARLA; BRENTANI, MARIA MITZI; et al. Down-regulation of ANAPC13 and CLTCL1: Early Events in the Progression of Preinvasive Ductal Carcinoma of the Breast. TRANSLATIONAL ONCOLOGY, v. 5, n. 2, p. 113-U105, APR 2012. (09/02457-2, 09/00669-2)