| Grant number: | 10/13836-1 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | March 01, 2011 |
| End date: | August 31, 2012 |
| Field of knowledge: | Biological Sciences - Botany - Pant Physiology |
| Principal Investigator: | Helenice Mercier |
| Grantee: | Alejandra Matiz Lopez |
| Host Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
Abstract Urease is a very important enzyme in the hydrolysis of urea, providing nitrogen to the plant. Previous studies developed in our Laboratory-IBUSP showed the preference of the epiphytic tank-bromeliad, Vriesea gigantea, for urea as a nitrogen source, and the localization of urease in the plasma membranes and cytoplasm of leaf cells. Recent evidences obtained in our laboratory linked a possible usage of the CO2 generated by hydrolysis of urea. Even with these results, it is not known yet if in a water shortage situation the plant would possess a preference even higher for the urea (reflecting in an increase of urease activity), as in this situation the stomata are closed and the entrance of the atmospheric CO2 into the plant is limited. This study will analyze the photosynthetic state, urease activity and CO2 cytolocalization in detached leaves of Vriesea gigantea under water shortage. | |
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