ULTRAWEAK LIGHT EMISSION DETECTION IN Giardia spp

• Grant number: 10/07792-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2011
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Agricultural Engineering - Soil and Water Engineering
• Principal Investigator: José Euclides Stipp Paterniani
• Grantee: Samuel Ricardo dos Santos
• Host Institution: Faculdade de Engenharia Agrícola (FEAGRI). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 13/01501-3 - Ultra-weak light emission in protozoa, BE.EP.DR

Abstract

This work aims to develop studies of photonics technique for detecting ultraweak light emission involving environmental parasitology and correlations with the viability of Cryptosporidium and Giardia. The methodology will be based on photon-counting measurements inside a dark-chamber with photomultiplier (PMT Hamamatsu H7360) with data acquisition performed by plate count (Hamamatsu M874), using suspensions of purified Cryptosporidium spp and Giardia spp. The results will be statistically analyzed based on the sum, average, standard deviation and frequency of photon-counting, comparing with the enumeration according to USEPA methods 1622 and 1623. With this study, it is estimated implement operational facilities and lead to short-term analysis in the viability of these protozoa.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, SAMUEL RICARDO; BRANCO, NILSON; BUENO FRANCO, REGINA MAURA; STIPP PATERNIANI, JOSE EUCLIDES; KATSUMATA, MASAKAZU; BARLOW, PETER W.; GALLEP, CRISTIANO DE MELLO. Fluorescence decay of dyed protozoa: differences between stressed and non-stressed cysts. LUMINESCENCE, v. 30, n. 7, p. 1139-1147, NOV 2015. (10/07792-1, 11/10763-6, 13/01501-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Samuel Ricardo dos. Delayed fluorescence in protozoa: Giardia intestinalis and Cryptosporidium parvum. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Agrícola Campinas, SP.