Cellular interactions in three-dimensional environment between dendritic cell-tumor cell hybrids and human lymphocytes: in search of strategies for enhancing antitumor vaccines

• Grant number: 10/18139-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2011
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Jose Alexandre Marzagão Barbuto
• Grantee: Karen Steponavicius Piedade Cruz
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 09/54599-5 - Dendritic cells: integrative elements of the immune system - an applied approach, AP.TEM

Abstract

Dendritic cell-tumor cell hybrids have been used in therapeutic vaccines for patients with advanced metastatic cancer, achieving promising results. However, both the behavior and the functions performed by these dendritic cell-tumor cell hybrids are still poorly understood, hindering its improvement. In this context, this project aims to study the interaction in vitro of these hybrid cells with different T lymphocyte subsets (CD4+, CD4+FoxP3+, CD4+FoxP3- and CD8+) in a three-dimensional environment. This setting allows cellular migration and the establishment of interactions in a selective way. The project will study also the pattern of lymphocyte responses by analyzing cytokine production by different cells. The main methodology to be employed will be confocal microscopy, and cell-cell interactions, after different time intervals, will be determined by differential staining of lymphocyte subsets. The presence of intracellular cytokines (IFN-gama, TGF-beta, TNF-alpha, IL-2, IL-4, IL-10 and IL-12) in these cells will also be analyzed. Time-lapse microscopy will be used to confirm the kinetics of the major cellular interactions. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

STEPONAVICIUS-CRUZ, KAREN; FREITAS, VANESSA MORAIS; BARBUTO, JOSE ALEXANDRE M.; BARTOLO, P; FERNANDES, P. Dendritic cells and T lymphocytes interactions in a novel 3D system. X INTERNATIONAL CONFERENCE ON STRUCTURAL DYNAMICS (EURODYN 2017), v. 59, p. 8-pg., 2013-01-01. (10/18139-7, 09/54599-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CRUZ, Karen Steponavicius Piedade. Cell interactions in three-dimensional environment between dendritic cell-tumor hybrid cells and human lymphocytes: looking for antitumor vaccine enhancement strategies.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.