Cell interactions in three-dimensional environment between dendritic cell-tumor hybrid cells and human lymphocytes: looking for antitumor vaccine enhancement strategies.

Author(s)

Karen Steponavicius Piedade Cruz

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-07-30

Examining board members

Jose Alexandre Marzagao Barbuto; Niels Olsen Saraiva Câmara; Fábio Martins Laginha; Elaine Guadelupe Rodrigues; Sandra Coccuzzo Sampaio Vessoni

• Advisor: Jose Alexandre Marzagao Barbuto; Vanessa Morais Freitas

Abstract

Dendritic cell-tumor cell hybrids have been used in therapeutic vaccines against cancer and has shown promising results, but their behavior and functions are insufficiently known. In this context, this study aims the interaction of these hybrid cells with different subpopulations of T cells in a 3D environment with collagen type III. Our results indicated that cells in Tumor Fusion group interacted with both CD4 and CD8 T lymphocytes. CD4 lymphocytes interacted 2 times more in the SKBR-3 Fusion group than another groups and their interaction was only with a single lymphocyte. CD8 lymphocytes interaction with the Tumor Fusion group resulted in longer interactions when in comparison with the another groups. No IL-2 was observed in cultures where the hybrid cells were present and the proliferation of lymphocytes in Tumor Fusion group was not observed. Thus, the 3D co-culture was able to demonstrate the importance of a custom protocol for each type of tumor, taking into consideration the unique characteristics of each one. (AU)

• FAPESP's process: 10/18139-7 - Cellular interactions in three-dimensional environment between dendritic cell-tumor cell hybrids and human lymphocytes: in search of strategies for enhancing antitumor vaccines
• Grantee: Karen Steponavicius Piedade Cruz
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)