There are mainly two Dendritic cells (DCs) subsets in murine spleen, one of them express the endocitic receptor CD205 (DEC205) and the alpha chain of CD8 receptor (DEC205+CD8±+) and the other one express the endocitic receptor DCIR2 but not CD8± (DCIR2+CD8±-). DEC205+CD8±+ DC subset is specialized in antigen cross presentation and CD8+ T cells priming and DCIR2+CD8±- DCs is associated with priming of CD4+ T cells. Targeting antigen to these two DCs subsets via monoclonal antibodies (mAbs) linked with antigenic proteins has been demonstrating good results as an efficient strategy to enhance the immunogenicity of recombinant proteins. Nevertheless, mechanisms of activation of humoral immune response as well as cell interactions induced by theses strategy of vaccination has not been completely clear yet. Thus, this study aims analyses humoral immune response and cell interactions among DCs, T and B cells induced by targeting a model antigen to two DCs subsets. We will use a chimeric mAbs fused in a hybrid protein, which is a portion of 19kDa of merozoite surface protein 1 (MSP119) expressed by Plasmodium vivax associated with PADRE (Pan allelic DR epitope) linked at a quimeric mAbs. The generation of immune response induced by these immunization protocol, the stimulus of booting antigen in enhancement and the influence of cells that express DEC205 or DCIR2 in antibodies will be analyzed. The study of theses mechanisms can help in optimization of vaccines to increase protein antigens immunogenicity.
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