Mechanism of generation of humoral immune response induced by targeting of MSP119PADRE antigen to two different subsets of dendritic cells via DEC205 and DCIR2 receptors.

Author(s)

Fernando Bandeira Sulczewski

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-11-24

Examining board members

Silvia Beatriz Boscardin; Jose Alexandre Marzagao Barbuto; Alexandre de Castro Keller; Maria Regina D'Imperio Lima

• Advisor: Silvia Beatriz Boscardin; Daniela Santoro Rosa

Abstract

As dendritic cells (DC) are innate immune cells that are specialized to prime adaptive immune cells. In the murine spleen, conventional DCs can be classified into CD8α+ DEC205+ and CD8α-DCIR2+. Monoclonal antibodies (mAbs) αDEC205 and αDCIR2 (33D1) conjugated to antigenic proteins have benn used as antigen targeting strategy to CD8α+ and CD8α- DCs. MSP119PADRE chimeric antigen conjugated to mAbs αDEC205 and αDCIR2 and Poly I: C as adjuvant was used. The assembly of the cellular and humoral response was analyzed 3, 4, 5 and 6 days after the first and second doses of imnunization. DCs CD8α- are specialized in the instruction of TFH cells. However, there is an a promotion of Th1 response by CD8α+, indicating that these cells are specialized in the instruction of the helper response profile. Nevertheless, in the second immunization there is a differentiation of TFH cells. (AU)

• FAPESP's process: 15/18874-2 - Mechanism of Generation of humoral immune response induced by targeting of MSP1(19)-PADRE antigen to two different subsets of dendritic cells via DEC205 and DCIR2 receptors
• Grantee: Fernando Bandeira Sulczewski
• Support Opportunities: Scholarships in Brazil - Master