EFFECT OF CHLORHEXIDINE, EPIGALLOCATECHIN-3-GALLATE AND CAFFEIC ACID PHENETHYL ESTER ON THE PRODUCTION AND EXPRESSION OF MATRIX METALLOPROTEINASE (MMP)-1, MMP-9 AND MATRIX METALLOPROTEINASE INHIBITOR-1 AFTER ACTIVATION BY LIPOPOLYSACCHARIDE

• Grant number: 10/18093-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2011
• End date: September 30, 2013
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Antonio Olavo Cardoso Jorge
• Grantee: Polyana das Graças Figueiredo Vilela
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The knowledge of the regulation factors of synthesis and secretion of MMP and TIMP is important for understanding the pathogenesis of pulp inflammation. Moreover, the role of MMP in tissue destruction has been evident in many diseases; therefore, there is intense interest to control its activities by pharmacological therapeutic. Thus, it is important to evaluate the effect of chlorhexidine (CHX), epigallocatechin-3-gallate (EGCG), caffeic acid phenethyl ester (CAPE) on gene expression of MMP-1, MMP-9 and TIMP-1, on the production of MMP-1 and on activity of MMP-9 by human monocytes after activation by lipopolysaccharide (LPS). For this study, human monocyte (THP-1) culture will be used. At first, the cell viability under different concentration of the material (CHX, EGCG, CAPE and LPS) will be evaluated by CellTiter 96® AQueous reagent assay. Then, non-cytotoxic concentration of CHX, EGCG e CAPE will be used to treatment the cells after activation by LPS. After 24 hours of treatment, the effect of CHX, EGCG and CAPE on the production of MMP-1 will be evaluated, whereas the gelatinolytic activity of MMP-9 will be assayed by gelatin zymography. In addition, by real-time PCR (qRT-PCR), the effect of the material on gene expression of MMP-1, MMP-9 and TIMP-1 by human monocytes will be evaluated. The results will be statistically analyzed by ANOVA with significance level of 5%, and the Tukey test.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIGUEIREDO VILELA, POLYANA DAS GRACAS; DE OLIVEIRA, JONATAS RAFAEL; DE BARROS, PATRICIA PIMENTEL; PEREIRA LEAO, MARIELLA VIEIRA; DE OLIVEIRA, LUCIANE DIAS; CARDOSO JORGE, ANTONIO OLAVO. In vitro effect of caffeic acid phenethyl ester on matrix metalloproteinases (MMP-1 and MMP-9) and their inhibitor (TIMP-1) in lipopolysaccharide-activated human monocytes. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 9, p. 1196-1202, SEP 2015. (10/18093-7)