Evaluation of the radiopacity, hydroxyl ion release and antimicrobial activity of Portland Cement associated with the zirconium oxide and niobium oxide nanoparticle

• Grant number: 10/16935-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2011
• End date: February 29, 2012
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Juliane Maria Guerreiro Tanomaru
• Grantee: Inara Storto
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The Mineral Trioxide Aggregate (MTA) presents on the basic composition Portland cement (PC). The association of zirconium oxide (OZ) or niobium oxide (iNO) as micro or nanoparticle substeances to CP can provide radiopacity and biological activity. It is important to analyze its properties. The aim of this study is to evaluate the radiopacity, the release of hydroxyl ions and antimicrobial activity of different endodontic materials as following: 1) white Portland cement (PC), 2) white MTA (Angelus), 3) + 30% CP Micro 4 OZ ) CP + 30% nanoparticulate OZ, 5) + 30% CP and micro ONi 6) CP + 30% nanoparticulate ONi. For the test will be made radiographs of standardized specimens in occlusal radiographs with a scale of aluminum increasing from 2 to 16 mm. The radiographic images will be digitized and analyzed in image software. The pH change will be evaluated with a pH meter. Tubes filled with material will be immersed in vials containing distilled water, and evaluated in experimental periods of 1, 7, 14, 21 and 28 days after the immersion of specimens in distilled water. The antimicrobial activity will be evaluated by agar diffusion test in triplicate. The microorganisms tested will be: Enterococcus faecalis (ATCC-29212), Streptococcus mutans (ATCC-25175), Pseudomonas aeruginosa (ATCC-27853), Micrococcus luteus (ATCC-9341) and Candida albicans (ATCC-10231). After incubation for 24 hours the inhibition area will be measured after the use of gel TTC. The data obtained in tests of radiopacity, pH and antimicrobial activity will be evaluated by proper statistical tests, taking p = 0.05. (AU)