Proteomics of fluoride effect in the in vitro osteogenic differentiation

• Grant number: 10/17658-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2011
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Camila Peres Buzalaf
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Fluoride (F) in micromolar doses enhances bone formation by up-modulating osteoblasts proliferation in vitro and in vivo. Experimental tests, using both humans and animals, have demonstrated the therapeutic effect of F in osteoporosis. However, its efficacy is still unclear based on the fact that part of osteoporotic patients who were treated with F did not respond positively. It suggested that other fators could be involved in the susceptibility to its effects. In this context, recently it was shown that different mice strains present variations in bone response to F. A/J mice bones present altered mecanical characteristics while bones of 129P3/J mice are not affected by F treatment, making them an appropriated in vivo model to highlight the molecular effects involved in the bone susceptibility and resistence to F. Resusts obtained by our group have been shown that F modulates the osteogenic differentiation of A/J and 129P3/J-derived mesenchymal cells in a dose and strain-dependent manner. Althought many biological mechanisms may be involved in this response, little is known about the impact of F on the modulation of proteins profile during several phases of the osteogenic differentiation in vitro. In this work, we sought to evaluate the proteomics of MsC obtained from AJ and 129P3/J in presence of F during the proliferation, differentiation and mineralization phases.