Effects of low pH and hypoxia induced by in vitro exposure to carbon dioxide on expression of genes related to apoptosis and proliferation in human peritoneum mesothelial cells

• Grant number: 11/02141-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2011
• End date: December 31, 2011
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Ângela Mara Bentes de Souza van Nimwegen
• Grantee: Mayara Moreno de Almeida
• Host Institution: Faculdade de Medicina do ABC (FMABC). Organização Social de Saúde. Fundação do ABC. Santo André , SP, Brazil

Abstract

The laparoscopic surgery includes a range of modern so-called "minimally invasive procedures". Most procedures performed by laparotomy can be performed by laparoscopy, which offers several advantages over conventional procedures including lower morbidity and shorter postoperative hospital stay, more rapid return to normal daily activities besides reduced trauma and local and systemic inflammatory responses mitigation. Despite the numerous advantages compared to open surgery, the need of co2-pneumoperitoneum results in negative responses such as increased intra-abdominal pressure, decrease in pH of peritoneal fluid with reduced macrophages reduced activity, temperature loss, desiccation of the peritoneal surface layer, wich leads to sub-endothelial layer exposure and oxidative stress, which can damage visceral and peritoneal mesothelial cells. Oxidative stress is involved in the pathogenesis of several diseases, including adhesion formation. The factors and cellular mechanisms involved in adhesion formation due to laparoscopic surgery are not well defined. The project goal is to investigate the influence of acidic pH and hypoxia induced by in vitro CO2-penumoperitoneum on proliferative and apoptotic genetic profile of mesothelial cells isolated from human peritoneum. (AU)