BIOMODULATION STUDY OF HUMAN EMBRYONIC STEM CELLS EXPOSED TO LOW POWER LASER IRRADIATION

• Grant number: 11/02469-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2011
• End date: December 31, 2011
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Newton Soares da Silva
• Grantee: Juliana Freires Mangolin
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

In 1998, Wisconsin University researcher James Thomson, and John Hopkins University researcher John Gearhart made history as the first scientists to cultivate human embryonic stem cells in vitro. On the 2nd of October of 2008, geneticist Lygia da Veiga Pereira of the university of Sao Paulo (USP), announced the development of the first line of human embryonic stem cells in Brazil, the BR-1 line. Advances in stem cell research have revealed the importance of these cells and their use in tissue engineering and even in the comprehension of active or inhibited epigenetic mechanisms in cell differentiation. Currently, studies related to proliferation, cell differentiation and epigenetic mechanisms use chemicals or exogenous growth compounds to activate these signals. Low intensity lasers of specific wavelengths have the capacity to induce the proliferation of different types of cells including human gum tissue fibroblasts, peripheral blood lymphocytes and HeLa cells. We intend through this project evaluate the use of low intesntisty laser (685 and 830nm) in varied densities as an alternative means in the process of proliferation and differentiation having as a base the expression of surface markers in the specific case of osteoblasts.