Purification of dextranasacarase by Leuconostoc mesenteroides FT045B hiring complete factorial planning

• Grant number: 11/05814-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2011
• End date: May 31, 2012
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Jonas Contiero
• Grantee: Franciele Lima de Oliveira
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The biopolymer dextran is formed by a homopolissacarídeo D-glucopyranosyl units, the types of bonds present vary according to the producer microorganism (BROW, Mark Avoy, 1990). Most of Dextran is synthesized from sucrose by the enzyme dextranasacarase processed, which is secreted by the bacteria grouped in the family Lactobacillaceae, more specifically in the genera Lactobacillus, Streptococcus and Leuconostoc, but the strain most widely used in research and is industrially Leuconostoc mesenteroides NRRL B512F (ROBYT, 1985, Takagi et al., 1994, ROBYT et al., 1979, Alsop, 1983, JEAN et al., 1954, and LINDBERG et al., 1968). The growing interest in specific applications of dextran is not due solely to the fact it is a product with hydrophilic characteristics, being stable and pure form and abilities of clear and stable solutions, but also because of the dextran to be derived from renewable resources and is biodegradable (PHARMACIA BIOTECH, 1997). The objective of this work is to study the purification of dextranasacarase employing complete factorial planning with two variables (concentration of PEG 1500 and 40000), the data will be analyzed by response surface methodology and multiple regression.