Characterization of anti-inflammatory action of the Melaleuca oil components(terpinen-4-ol e alpha-terpineol) in human macrophages activated by lipopolysaccharide of Porphyromonas gingivalis

• Grant number: 10/18968-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2011
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Marianne Nicole Marques Nogueira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Periodontal disease is essentially an inflammatory disease where the host defense system makes use of chemical mediators in controlling the inflammatory process producing circulatory changes with defensive cells, besides acting directly on periodontopathic bacteria. However, the host response can vary from individual to individual, and can activate the destruction of periodontal support and protection of one or more teeth. Mechanical removal as a way to control the biofilm is well established as a preventive activity, and use of chemical control are presented as an aid in this process. However, modulation of the host response that attenuates exacerbated by controlling the production of cytokines, it has become today. In this context, natural extracts have demonstrated therapeutic effects both in vitro and in vivo. The essential oil of Melaleuca alternifolia was used as an antimicrobial agent or preventive scale pharmaceutical or cosmetic and indications ranging from the use in wounds, such as gynecological cream and even toothpaste. Added to this, it is suggested that the Melaleuca alternifolia characteristics are anti-inflammatory properties, especially its soluble portion, inhibiting response to lipopolysaccharide in the production of interleukin-1B, the factor-± tumor necrosis factor, interleukin-10. Thus, the objective of this research is to study the molecular mechanisms involved in anti-inflammatory activity of oil of Melaleuca alternifolia, for both experiments will be conducted to assess and quantify the expression of some genes involved in this process as well as production of some cytokines.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA, M. N. M.; AQUINO, S. G.; ROSSA JUNIOR, C.; SPOLIDORIO, D. M. P.. Terpinen-4-ol and alpha-terpineol (tea tree oil components) inhibit the production of IL-1 beta, IL-6 and IL-10 on human macrophages. Inflammation Research, v. 63, n. 9, p. 769-778, SEP 2014. (10/18968-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOGUEIRA, Marianne Nicole Marques. Caracterização da ação modulatória de citocinas inflamatórias pelo óleo de Melaleuca alternifolia e seus componentes (terpinen-4ol e alfa-terpineol) em macrófagos humanos ativados lipopolissacarídeos de Porphyromonas gingivalis e Escherichia coli. 2013. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.