Resveratrol action on primary and secondary cartilages of the undernourished and refed wistar rats

• Grant number: 11/12746-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2011
• End date: July 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Edson Aparecido Liberti
• Grantee: Joice Naiara Bertaglia Pereira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Resveratrol is a polyphenol contained in grapes that has osteogenic action among other numerous beneficial to the body. It is known that malnutrition affects craniofacial growth. So this study aims to assess the effects of resveratrolon primary and secondary cartilage in two important sites responsible for craniofacial growth, respectively, the basioccipital synchondrosis (SBO) and the mandibular condyle(PC). For both experimental groups will be formed of specimensmalnourished pre-and post-natal (Group D) and fednormally (Group N) with 21 and 60 days (Groups NN and DD) among which is the suplemetado with resveratrol (Group RRV), renourish to be the starting day 21. Their cartilage SBO and PC will be obtained histological sections of 5 mm to beused for staining picrosirius and azo-carmine, thewhich collagen and cellular components are shown. the sections will also be submitted to immunohistochemical reaction identification of the growth factor similar to insulin, IGF-1, and its receptor, as expressing the action of cartilage growth hormone. The qualitative results are complemented by quantitative, which involve taking parameters such as cartilage thickness of the layers and number ofreactive cells. For the final evaluation, the parameters are subjected to analysis of variance with one factor, followed by multiple comparisons by the method of Scott Knott. (AU)