Quantitative analysis and Immunohistochemistry evaluation of inflammatory reaction from monofilamentar polypropylene mesh coated with S-nitrosoglutathione-releasing poly(vinyl alcohol) film in grafted in subcutaneous rats

• Grant number: 11/18026-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2011
• End date: October 31, 2012
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Cássio Luís Zanettini Riccetto
• Grantee: Rafael Miranda da Costa
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Introduction: The use of polypropylene mesh to repair urinary incontinence and vaginal prolapse has decrease recurrence and nowadays it became a very common option. However, it has also been related to specific complications such as vaginal erosions. Nitric Oxide can modulate angiogenesis, vasodilation and fibroblastic reaction. Experimentally, meshes coated with nitric oxide showed more angiogenesis and less edema. Objective: To study, quantitatively, the effect of S-nitrosoglutatione in integration of monofilamentar polypropylene mesh in grafted in subcutaneous rats. Methods: Thirty adult female Wistar rats will be used. Five mesh fragments measuring 10 x 10 mm will be implanted in subcutaneous tissue of each rat. Polyprolylene fragments will be prepared as follows: without coating; coated with poly(vinyl alcohol) (PVA); coated with PVA + S- Nitrosglutatione (GSNO)1mM; coated with PVA + S-Nitrosglutatione (GSNO)10mM; coated with PVA + S-Nitrosglutatione (GSNO)70mM. Fifteen rats will be euthanized at 4 days and the other at 30 days postoperatively. The inflammatory reaction area after four days and fibroblastic reaction area after 30 days will be measured in hematoxin-eosin stained glasses with the image analyzer AxioVision®. A polarization microscope will be used to collagen type identification and fiber orientation study. The molecular evaluation of NO syntetase activity, cellular toxicity, angiogenesis and collagen degradation will be with immunohistochemistry and Western Blot.