MESOPOUROS SILICA NANOPARTICLES AS A STRATEGY IN ANTITUMORAL THERAPY

• Grant number: 11/17195-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2011
• End date: October 31, 2014
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Marcelo Lancellotti
• Grantee: Luciana Maria de Hollanda
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Cancer is one of diseases mainly caused by genetic changes that give cells, unlimited capacity of proliferation, inability to enter apoptosis, metastasis and angiogenesis. These factors are often originated by an imbalance in the expression of certain groups of proteins. In many tumors the protein Bcl-2 related apoptosis is hiperexpressa. Thus, it is essential that procedures are performed to decrease the expression of this protein. To make this feasible, it is common to use RNAi. As this is easily degraded it is customary to combine it with vectors. In recent years, there was great commitment of the international scientific community for these vectors were nanoparticles. Among all nanovetores used mesoporous silica nanoparticles showed higher capacity of cellular penetration. Moreover, being porous, having ordered channels, large surface areas and high compatibility with drugs were tested as carriers of drugs and genes, and RNAi drugs and with great efficiency and success.Thus, we will test whether the mesoporous silica nanoparticles produced by Prof.. Edessa Dr. Martins de Souza Barros (CDTN-CNEN), are jointly capable of carrying Bcl-2 and RNAi decreasing the amount of tumor cells in vitro models. In addition, we evaluate the reduction in the expression of this important protein by Real Time PCR. Still, compare that with the decrease in mRNA expression of the alveolar cells of basal cell adenocarcinoma of human lung (A549), human lung carcinoma large cell (NCI-H460), human pancreatic carcinoma (PANC-1) and human glioblastoma (NG97), immortalized human keratinocyte (HaCaT) alter their pattern of apoptosis, angiogenesis, metabolism, and drug resistance by the methodology of PCR Array. So invest in this area is of paramount importance, since new scientific frontiers will be opened, raising the status of the course and introducing them to one of the most relevant and most prestigious international nanobiotechnology is to treat tumor.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOLLANDA, L. M.; LOBO, A. O.; LANCELLOTTI, M.; BERNI, E.; CORAT, E. J.; ZANIN, H.. Graphene and carbon nanotube nanocomposite for gene transfection. Materials Science & Engineering C-Materials for Biological Applications, v. 39, p. 288-298, JUN 1 2014. (11/17195-3, 11/17877-7)

ZANIN, H.; HOLLANDA, L. M.; CERAGIOLI, H. J.; FERREIRA, M. S.; MACHADO, D.; LANCELLOTTI, M.; CATHARINO, R. R.; BARANAUSKAS, V.; LOBO, A. O.. Carbon nanoparticles for gene transfection in eukaryotic cell lines. Materials Science & Engineering C-Materials for Biological Applications, v. 39, p. 359-370, JUN 1 2014. (11/17195-3, 11/17877-7)

FERREIRA, T. H.; HOLLANDA, L. M.; LANCELLOTTI, M.; DE SOUSA, E. M. B.. Boron nitride nanotubes chemically functionalized with glycol chitosan for gene transfection in eukaryotic cell lines. Journal of Biomedical Materials Research Part A, v. 103, n. 6, p. 2176-2185, JUN 2015. (11/17195-3)