Ex vivo evaluation of the effectiveness of different techniques for the removal of calcium hydroxide from root canal

• Grant number: 11/18922-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: November 30, 2012
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Gisele Faria
• Grantee: Kennia Scapin Viola
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Calcium hydroxide [Ca (OH)2] residues in root canals may compromise the sealing ability of the sealers and consequently the success of endodontic treatment. This study will compare the effectiveness of different techniques for removal of Ca(OH)2 from root canal, through the analysis of images in scanning electron microscope (SEM). Sixty-six extracted human canines will be instrumented with the ProTaper rotary system to the instrument F5, under irrigation with 2.5% NaOCl. Then the root canals will be irrigated with 5 mL of 2.5% NaOCl, 5 mL of 17% EDTA, dried and filled with Ca (OH)2 which will remain in the canal for 7 days. The specimens will be distributed into 4 groups (n=15) according to the Ca(OH)2 removal technique. Irrigation with conventional needle and rotary master apical file will be used in GI (control). Irrigation with conventional needle and rotary master apical file will be used in the other three groups, followed by the use of EndoVac Plus (GII), EndoActivator (GIII) and passive ultrasonic irrigation (GIV). It will be employed 15 mL of 2.5% NaOCl and 3 mL of 17% EDTA during the Ca (OH)2 removal in all groups. Three canines will receive Ca(OH)2 paste, but no subsequent removal (positive control) and 3 will receive no Ca(OH)2 (negative control). The teeth will be prepared for analysis by SEM. The amount of Ca (OH)2 residues will be evaluated in the apical and middle thirds of the root canal using a 5-grade scoring system by 2 examiners. The results will be analyzed by the nonparametric Kruskal-Wallis and Dunn tests, with a significance level of 5%.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARIA, GISELE; VIOLA, KENNIA SCAPIN; KUGA, MILTON CARLOS; ARANDA GARCIA, ARTURO JAVIER; DAHER, VANESSA BOSSOLANI; DE PASQUALI LEONARDO, MARIO FRANCISCO; TANOMARU-FILHO, MARIO. Effect of Rotary Instrument Associated with Different Irrigation Techniques on Removing Calcium Hydroxide Dressing. MICROSCOPY RESEARCH AND TECHNIQUE, v. 77, n. 8, p. 642-646, AUG 2014. (11/18922-6)