GENOTOXIC AND MUTAGENIC EFFECTS OF ALLYL ISOTHIOCYANATE IN BLADDER CANCER CELLS.

• Grant number: 11/19618-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: December 31, 2012
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Daisy Maria Favero Salvadori
• Grantee: André Luiz Ventura Sávio
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Cancer is a serious public health problem. Particularly, bladder cancer represents oneof the tumors of high cost for health systems, especially because of routine clinicalmonitoring (urine cytology and cystoscopy) and high recurrence. Currently, it is frequent the number of natural products with therapeutic properties used by the population. Among these, the alkyl isothiocyanate (AITC), compound present in mustard seed and cruciferous vegetables, is commonly studied. Some authors have shown that the AITC leads to cell cycle arrest and apoptosis in some types of cancer cells, including prostate and colon. In the bladder, the rapid absorption and high bioavailability make the AITC as a promising agent for the treatment and prevention of urothelial cancer. Thus, this project aims investigate the anti-proliferative, cytotoxic, mutagenic and genotoxic potential of allil isothiocyanate in two bladder transitional cell carcinoma (TCC) cell lines: RT4 - tumor grade I and T24 - grade III tumor. For those evaluations clonogenic survival assay, XTT test, comet (Single Cell Gel) and the micronucleus assays will be used. It is expected that the results may contribute to the understanding of action mechanism of AITC, which has promising activity against bladder cancer.