GENE EXPRESSION OF TOLL-LIKE RECEPTORS IN CULTURE OF DERMAL FIBROBLASTS OF BURNED PATIENTS

• Grant number: 11/18384-4
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2011
• Effective date (End): November 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alfredo Gragnani Filho
• Grantee: Marcus Vinicius Boaretto Cezillo
• Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Introduction: The burn causes damage to cell membranes, resulting in a dynamic cascade of inflammatory mediators that exacerbate the regulation of systemic inflammatory responses. Cytokines are the primary mediators of this inflammatory response to thermal injury by promoting communication between various types of cells. The gene expression of cytokines and growth factors of the fibroblasts are associated with the stress condition of the environment in which the cells are subjected. Given the correlation between changes in the extracellular environment and changes in transcriptional profile of fibroblasts, there is change of genes involved in innate immunity and signal transduction mediated by Toll-like receptors in the skin fibroblasts inflammatory response to burning of large body surface. Objective: To evaluate the expression of 84 genes involved in the signal transduction mediated by Toll-like receptors in cultured dermal fibroblasts from patients with burn injury. Methods: The skin feasible fragments, from devitalized margins of skin, will be subjected to homogenization. The RNA obtained is isolated and converted into cDNA for analysis by PCR array. Amplifications of 84 genes involved in innate immunity and signaling transduction through Toll-like receptors will be evaluated. Gene expression will be considered regulated up or down-regulated, comparing it to the established standard.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRAGNANI, ALFREDO; CEZILLO, MARCUS V. B.; DA SILVA, ISMAEL D. C. G.; RIBEIRO DE NORONHA, SAMUEL M.; CORREA-NORONHA, SILVANA A. A.; FERREIRA, LYDIA M. Gene expression profile of cytokines and receptors of inflammation from cultured keratinocytes of burned patients. BURNS, v. 40, n. 5, p. 947-956, AUG 2014. Web of Science Citations: 15.