Mathematical model of action potential and Ca2+ transport in neonatal rat ventricular myocyte.

• Grant number: 11/19805-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2012
• End date: March 31, 2014
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Jose Wilson Magalhaes Bassani
• Grantee: Natalia Ferreira Oshiyama
• Host Institution: Centro de Engenharia Biomédica (CEB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Mathematical models of the cardiac action potential (AP) are useful to help understand the overall electrophysiology of cardiac myocytes and to predict how changes in ion currents may affect the cardiac electrical activity. If AP models are integrated to those that describe the cell Ca2+ dynamics, it is possible to gain better insight into the excitation-contraction coupling phenomenon. Cardiac myocytes from immature mammals differ functionally from adult cells, not only in the electrophysiological aspect, but also in the Ca2+ dynamics. Despite the large utilization of neonatal rat cardiomyocytes, there are only few models that are specific for this type of cell. In this work, it is proposed to develop an integrated model of AP and Ca2+ transport in neonatal rat ventricular cells, based on the existing models and on data gathered from our laboratory and literature. Moreover, it is also proposed to carry out experiments for measurement of the intracellular Na+ concentration and Na+ current in freshly isolated neonatal rat ventricular myocytes, which are data currently lacking in the literature.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OSHIYAMA, NATALIA F.; PEREIRA, ANA H. M.; CARDOSO, ALISSON C.; FRANCHINI, KLEBER G.; BASSANI, JOSE W. M.; BASSANI, ROSANA A.. Developmental differences in myocardial transmembrane Na+ transport: implications for excitability and Na+ handling. JOURNAL OF PHYSIOLOGY-LONDON, v. 600, n. 11, p. 17-pg., 2022-05-11. (11/19805-3)

ZAFALON, JR., NIVALDO; OSHIYAMA, NATALIA F.; BASSANI, JOSE W. M.; BASSANI, ROSANA A.. Muscarinic stimulation and pinacidil produce similar facilitation of tachyarrhythmia induction in rat isolated atria. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, v. 65, p. 120-126, DEC 2013. (11/19805-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OSHIYAMA, Natalia Ferreira. Mathematical model of action potential and Ca2+ transport in ventricular myocytes of neonatal rats. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Elétrica e de Computação Campinas, SP.