Effect of aroeira extract on adherence and proliferation of fibroblasts

• Grant number: 11/22243-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: December 31, 2012
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Rodrigo Cardoso de Oliveira
• Grantee: Patricia Fernandes Sartori
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aroeira's extract study and their effects in specific tissues and cells can contribute significantly to the understanding and validation of your property like herbal. The aim of this study is to evaluate the influence of aroeira's extract in human gingival fibroblast and the matrix metalloproteinase 2 and 9 activity. First, the fibroblasts will be plated with media Eagle modified by Dullbecco complement for 10% fetal bovine serum. After the fibroblasts adhesion, DMEM will be substituted in a culture medium with different aroeira hydroalcoholic extract concentrations (pure extract; 1:10; 1:100; 1:1000; 1:1000 and control). After 24, 48, 72, 96 and 120 hours of the treatment with the different extract concentration, the sample will be collected, shared, and analyzed. The collected samples will be available the cellular viability by cells mitochondrial activity test will be realized by the MTT's reduction method (bromate 3-(4,5-dimetiltiazol-2-yl)-2,5-difeniltetrazólio), neutral red capture and violet crystal. The other sample will be used to available the MMP 2 and 9 activity for zymography. The values will be evaluated by ANOVA's method, complemented by Dunn's test when submit to normal or abnormal distribution, respective. The significant level used will be 5% (p<0.05).(AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, ALESSANDRA CURY; SOUZA, LEONARDO PEREZ; SALDANHA, LUIZ LEONARDO; PIERONI, LAIS GOYOS; MATOS, ADRIANA ARRUDA; DE OLIVEIRA, FLAVIA AMADEU; VILEGAS, WAGNER; DAMANTE, CARLA ANDREOTTI; DOKKEDAL, ANNE LIGIA; DE OLIVEIRA, RODRIGO CARDOSO. ``Aroeira{''} (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects. Pharmaceutical Biology, v. 54, n. 11, p. 2737-2741, NOV 2016. (11/22243-7, 10/14039-8, 10/02026-9)

MACHADO, ALESSANDRA CURY; SARTORI, PATRICIA FERNANDES; DAMANTE, CARLA ANDREOTTI; DOKKEDAL, ANNE LIGIA; DE OLIVEIRA, RODRIGO CARDOSO. Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic ``Aroeira{''} Extract (Myracrodruon urundeuva Allemao). Brazilian Archives of Biology and Technology, v. 59, 2016. (11/22243-7, 10/02026-9)

MACHADO, ALESSANDRA CURY; SOUZA, LEONARDO PEREZ; SALDANHA, LUIZ LEONARDO; PIERONI, LAIS GOYOS; MATOS, ADRIANA ARRUDA; DE OLIVEIRA, FLAVIA AMADEU; VILEGAS, WAGNER; DAMANTE, CARLA ANDREOTTI; DOKKEDAL, ANNE LIGIA; DE OLIVEIRA, RODRIGO CARDOSO. "Aroeira" (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects. Pharmaceutical Biology, v. 54, n. 11, p. 5-pg., 2016-11-01. (11/22243-7, 10/14039-8, 10/02026-9)

ALESSANDRA CURY MACHADO; PATRÍCIA FERNANDES SARTORI; CARLA ANDREOTTI DAMANTE; ANNE LÍGIA DOKKEDAL; RODRIGO CARDOSO DE OLIVEIRA. Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão). Brazilian Archives of Biology and Technology, v. 59, 2016-03-08. (11/22243-7, 10/02026-9)