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Effect of the extract of aroeira in the process of proliferation and mineralization of human osteoblasts in vitro

Grant number: 10/14039-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): July 31, 2013
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Rodrigo Cardoso de Oliveira
Grantee:Adriana Arruda Matos
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Due to the indiscriminate use of antibiotics, the resistance of pathogenic microorganisms to various drugs has increased in recent years. This situation has forced researchers to search for new drugs. An alternative is the herbal medicines that are pharmaceutical preparations (extracts, tinctures, ointments and capsules) of medicinal herbs, used to treat numerous diseases. The advance is among the plants with antimicrobial and antifungal. One of the Brazilian species of woodland that have shown interest in various areas is the aroeira, by presenting anti-inflammatory action. Osteoblasts are young cells with intense metabolic activity and responsible for producing the organic part of the matrix. The activity of osteoblasts depends on the actions of some modulators such as growth factors such as Bone Morphogenetic Proteins (BMPs), cytokines, matrix metalloproteinases (MMPs), alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP), among others. The aim of this study is evaluate the effect of the extract of aroeira on the proliferation and mineralization of human osteoblasts and whether there is any relationship between the effect of the extract with the expression of MMP-2 and -9; Osteopontin (OPN), bone sialoprotein (BSP), BMP-2 and -7 and Human Ankylosis (ANKH) produced by these cells. For the experiment will be collected aroeira in the campus of UNESP, and you will be extracted from hydroalcoholic extract of aroeira. Then will be cultivated human osteoblasts in ±-MEM medium supplemented with 10% Fetal Bovine Serum (FBS). The experimental groups will be divided into different concentrations of the hydroalcoholic extract of aroeira (pure extract, 1: 10, 1:100, 1:1000, 1:10000). The analysis of cell viability will be done by Uptake of Neutral Red, Reduction of MTT and Crystal Violet, to analyze the expression of MMP-2 and -9, OPN, BSP, BMP-2 and -7, and ANKH through RT- PCR and Western blot. The statistical analyses will be made through the statistical test one-way ANOVA complemented for the test of Tukey, test of Kruskal-Wallis complemented for the test of Dunn and for the "t" test, according to the indication (p <0.05). Keywords: Osteoblasts, Cell Culture, Extract Aroeira, Bone mineralization. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACHADO, ALESSANDRA CURY; SOUZA, LEONARDO PEREZ; SALDANHA, LUIZ LEONARDO; PIERONI, LAIS GOYOS; MATOS, ADRIANA ARRUDA; DE OLIVEIRA, FLAVIA AMADEU; VILEGAS, WAGNER; DAMANTE, CARLA ANDREOTTI; DOKKEDAL, ANNE LIGIA; DE OLIVEIRA, RODRIGO CARDOSO. ``Aroeira{''} (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects. Pharmaceutical Biology, v. 54, n. 11, p. 2737-2741, . (11/22243-7, 10/14039-8, 10/02026-9)
MATOS, ADRIANA ARRUDA; OLIVEIRA, FLAVIA AMADEU; MACHADO, ALESSANDRA CURY; SALDANHA, LUIZ LEONARDO; TOKUHARA, CINTIA KAZUKO; SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; DIONISIO, THIAGO JOSE; SANTOS, CARLOS; PERES-BUZALAF, CAMILA; et al. An extract from Myracrodruon urundeuva inhibits matrix mineralization in human osteoblasts. Journal of Ethnopharmacology, v. 237, p. 192-201, . (10/02026-9, 10/14039-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MATOS, Adriana Arruda. Effect of aroira extract in the process of proliferation and mineralization of osteoblasts in vitro. 2013. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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