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Adhesion, proliferation and differentiation of preosteoblasts cultured on a laser treated titanium surface


Titanium is a widely used as a material for substitution of anatomic structures, being considered as the most common material employed in cases of osseointegrated implants. It has been described that the osseointegration, requisite for a good functionality of implants, is a slow process, sequentially characterized by the steps of adhesion, differentiation and cellular proliferation, as well as for apposition and mineralization of matrix deposited by osteoblasts. The bone quality and surface treatment of titanium have a direct influence on osseo integration process. Therefore, the aim of this study is to evaluate, in vitro, if the modification of the titanium surface with a high energy laser, could improve those biological processes of proliferation, differentiation, and metabolical activity on mouse calvarial pre-osteoblast. The cell metabolism will be analyzed by MTT test, the osteoblast phenotypical markers will be assessed by qPCR, enzymatic activity of alkaline phosphatase and mineralization nodules formation (Alizarin-red) will also be analyzed. The numeric data obtained from this proposed experimental protocol will be statistically analyzed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROBERTI GARCIA, LUCAS DA FONSECA; VOLTOLINI PONTES, ELAINE CRISTINA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; SOARES, DIANA GABRIELA. Transdentinal cytotoxicity of resin-based luting cements to pulp cells. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 7, p. 1559-1566, . (13/23520-0, 13/05879-0, 12/14912-9)
VOLTOLINI PONTES, ELAINE CRISTINA; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Cytotoxicity of resin-based luting cements to pulp cells. AMERICAN JOURNAL OF DENTISTRY, v. 27, n. 5, p. 237-244, . (12/14912-9)
MARISCAL-MUNOZ, EDUARDO; COSTA, CARLOS A. S.; TAVARES, HEWERSON S.; BIANCHI, JONAS; HEBLING, JOSIMERI; MACHADO, JOAO P. B.; LERNER, ULF H.; SOUZA, PEDRO P. C.. Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb:YAG laser irradiation. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 3, p. 503-511, . (11/16351-1, 12/14912-9)

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