Characterization of hypothalamic PTP1B activity in obese rodents submited to physical exercise.

• Grant number: 11/14727-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: July 31, 2013
• Field of knowledge: Biological Sciences - Physiology - Physiology of Effort
• Principal Investigator: José Rodrigo Pauli
• Grantee: Eloize Cristina Chiarreotto Ropelle
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

The food intake and energy expenditure are regulated by specific neurons in the hypothalamus. During the last two decades, the localization of insulin and leptin receptors in hypothalamic nuclei and the description of the intracellular signaling triggered by these hormones, were crucial to understanding the control of food intake and energy expenditure. The subclinical inflammation observed in experimental models of obesity is associated with distinct mechanisms of leptin and insulin resistance in the hypothalamus and result in increased food intake and body weight gain. Among these mechanisms, the hypothalamic Protein Tyrosine Phosphatase-1B (PTP1B) activity has been investigated. The tyrosine phosphatase PTP1B is able to dephosphorylate the insulin receptor (IR), the insulin receptor substrate 1 (IRS-1) that make up the cascade of insulin signaling, in addition PTP1B also dephosphorylates the Jannus kinase 2 ( Jak2), the protein responsible for transmitting the leptin signal. Recent studies have shown that PTP1B is a key molecule for insulin and leptin resistance in the hypothalamus, leading to increased food intake and body weight. On the other hand, exercise is able to restore insulin and leptin sensitivity in the hypothalamus of obese rodents through the mechanism involving interleukin-6 (IL-6). However, the effects of exercise on the hypothalamic PTP1B activity are unknown. Thus, the goal of this project is investigate the effect of exercise and IL-6 on the hypothalamic PTP1B expression and activity and relate these effects of insulin and leptin sensitivity in the hypothalamus and food intake. These findings may contribute significantly to the understanding of how exercise is associated to improvement of satiety signals in obesity state.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHIARREOTTO-ROPELLE, ELOIZE C.; PAULI, LUCIANA S. S.; KATASHIMA, CARLOS K.; PIMENTEL, GUSTAVO D.; PICARDI, PATY K.; SILVA, VAGNER R. R.; DE SOUZA, CLAUDIO T.; PRADA, PATRICIA O.; CINTRA, DENNYS E.; CARVALHEIRA, JOSE B. C.; et al. Acute exercise suppresses hypothalamic PTP1B protein level and improves insulin and leptin signaling in obese rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v. 305, n. 5, p. E649-E659, SEP 2013. (11/14727-4, 11/13779-0, 10/12091-2)

SOUZA PAULI, LUCIANA SANTOS; CHIARREOTTO ROPELLE, ELOIZE CRISTINA; DE SOUZA, CLAUDIO TEODORO; CINTRA, DENNYS ESPER; RAMOS DA SILVA, ADELINO SANCHEZ; RODRIGUES, BARBARA DE ALMEIDA; DE MOURA, LEANDRO PEREIRA; MARINHO, RODOLFO; DE OLIVEIRA, VANESSA; KATASHIMA, CARLOS KIYOSHI; et al. Exercise training decreases mitogen-activated protein kinase phosphatase-3 expression and suppresses hepatic gluconeogenesis in obese mice. JOURNAL OF PHYSIOLOGY-LONDON, v. 592, n. 6, p. 1325-1340, MAR 15 2014. (11/14727-4, 11/13779-0, 10/12091-2)

SOUZA PAULI, LUCIANA SANTOS; CHIARREOTTO ROPELLE, ELOIZE CRISTINA; DE SOUZA, CLAUDIO TEODORO; CINTRA, DENNYS ESPER; RAMOS DA SILVA, ADELINO SANCHEZ; RODRIGUES, BARBARA DE ALMEIDA; DE MOURA, LEANDRO PEREIRA; MARINHO, RODOLFO; DE OLIVEIRA, VANESSA; KATASHIMA, CARLOS KIYOSHI; et al. Exercise training decreases mitogen-activated protein kinase phosphatase-3 expression and suppresses hepatic gluconeogenesis in obese mice (Publication with Expression of Concern. See vol. 594, pg. 5027, 2016) (Publication with Expression of Concern. See vol. 594, pg. 5027, 2016) (Publication with Expression of Concern. See vol. 594, pg. 5027, 2016). JOURNAL OF PHYSIOLOGY-LONDON, v. 592, n. 6, p. 16-pg., 2014-03-15. (10/12091-2, 11/14727-4, 11/13779-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROPELLE, Eloize Cristina Chiarreotto. Characterization of hypothalamic PTP1B activity in obese rodents submited to physical exercise. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.