Metalloproteins characterization by electrospray ionization mass spectrometry (ESI-MS) in samples of Nile Tilapia (Oreochromis niloticus).

• Grant number: 11/14902-0
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2012
• Effective date (End): February 28, 2014
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Pedro de Magalhães Padilha
• Grantee: Bruna Cavecci Mendonça
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This study aims to evaluate the metalloproteomics profile of samples from Nile tilapia (Oreochromis niloticus) muscle tissue. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) will be used for the fractionation of proteins from the muscle tissue samples and for identification of these proteins by analyzing the images obtained by the end of the electrophoretic process. Calcium, Copper, Iron, Manganese and Zinc mapping in protein spots will be made by Synchrotron Radiation X-ray Fluorescence. Different methods for protein extraction and different programs for protein separation by 2D PAGE will be tested. The protein spots containing Ca, Cu, Fe, Mn and Zn will be characterized by ESI-MS. The characterization of these metalloproteins can be a springboard for the Nile tilapia's metallomics. It can also provide important information for the area of fish nutrition and physiology, bringing advances for the proteome and metallome study of this species.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVECCI, BRUNA; DE LIMA, PAULA M.; DE QUEIROZ, JOAO V.; BRAGA, CAMILA P.; PADILHA, CILENE C. F.; LEITE, ALINE L.; BUZALAF, MARILIA A. R.; PEZZATO, LUIZ E.; PADILHA, PEDRO M. Metalloproteomic Profile Determination of Muscle Samples from Nile Tilapia (Oreochromis niloticus) Using AAS and ESI-MS/MS after 2D-PAGE Separation. Journal of the Brazilian Chemical Society, v. 26, n. 2, p. 239-246, FEB 2015. Web of Science Citations: 2.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MENDONÇA, Bruna Cavecci. Caracterização por espectrometria de massas de metaloproteínas em amostras de Tilápia do Nilo. 2014. 60 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina Veterinária e Zootecnia. Botucatu.