Expression and localization of EGFR in non-small cell lung cancer: its role in proliferation and cell migration

• Grant number: 11/19040-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2012
• End date: January 19, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Glaucia Maria Machado Santelli
• Grantee: Camila Lauand
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The lung cancer cells frequently have changes in their molecular genetics, for example overexpression of ErbB1 gene. ErbB1 gene encoded EGFR, a receptor tyrosine kinase that is mainly responsible for cell proliferation and survival. EGFR Overexpression has been associated with more aggressive disease, poor prognosis, low survival and low response to therapy. There are many studies focused on the ErbB1 gene amplification, mutations and EGFR overexpression, but there are many controversies in the literature on these aspects. HK2 and A549 cell lines, derived from human lung carcinoma, have been used as models to study in our laboratory. Under these conditions we propose to characterize these two cells, in a 2D and 3D culture system, on the number of copies of gene ErbB1 gene and level of EGFR expression. The activation of EGFR will be inhibited by the tyrosine kinase domain inhibitor AG1478 to evaluate the influence of this receptor on the proliferation and cell migration in these cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LAUAND, C.; NIERO, E. L.; DIAS, V. M.; MACHADO-SANTELLI, G. M.. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells. Brazilian Journal of Medical and Biological Research, v. 48, n. 5, p. 382-391, MAY 2015. (11/19040-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LAUAND, Camila. Evaluation of cell proliferation and migration mediated by EGFR activation in lung cancer cell lines grown as monolayers and spheroids.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.