Is the expression of molecular markers for bovine spermatogonial stem cell diferent between pre-pubertal and adult animals?

• Grant number: 11/20115-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: June 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Antonio Visintin
• Grantee: Mariana Ianello Giassetti
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 12/05474-8 - YY2 target genes as molecular marker for SSC, BE.EP.DR

Abstract

Spermatogenesis in mammals is based on self-renewal and differentiation of spermatogonial stem cells (SSCs ), which are used for different research goals since restablishment of fertility in males until produce the production of animals transgenics. However, the knowledge about the cell biology of SSCs is not very well established and is most scarce for other species other than human or murine, such as bovine. Different factors, such as male's age, can affect the expression of many genes and a specific molecular markers for SSCs and these factors are still little understood. The lack of this knowledge is one of the main reasons that make the biotechnological processes and therapeutics manipulation of SSC inefficient. The hypotesis of present project is: the expression of molecular markers for SSCs purified from bovine pre-pubertal differs from adults. In this way the main goal is to characterize and to compare the gene expression and the presence of molecular markers for bovine SSCs in these two different ages.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIASSETTI, M. I.; GOISSIS, M. D.; DE BARROS, F. R. O.; BRUNO, A. H.; ASSUMPCAO, M. E. O. A.; VISINTIN, J. A.. Comparison of Diverse Differential Plating Methods to Enrich Bovine Spermatogonial Cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 51, n. 1, p. 26-32, FEB 2016. (12/05474-8, 13/03495-0, 11/20115-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GIASSETTI, Mariana Ianello. Expression of molecular markers in spermatogonia. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.