Synthesis and characterization of hemoglobin based oxygen carriers

• Grant number: 11/21933-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Bronislaw Polakiewicz
• Grantee: Marcos Camargo Knirsch
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The development of a product that is able to transport oxygen to the tissues and also to control hypovolemia on trauma patients (commonly known as blood substitutes) will cause a great impact on trauma treatment and on surgical interventions. Hemorrhage is the major cause of death after serious accidents and blood volume control is the major priority on such situations. Transfusion may also be impacted by such product because many complications are related to the therapeutic application of blood. The main issue related to the usage of blood is the risk of pathogenic agents transmission (such as HIV, HBV, HCV, CMV, etc.). Complications related to imunomodulation may also occur with the therapeutic use of blood thus increasing the risk of infection as well as acute lung injury and severe hemolytic reactions. In addition concern arises from the disparity between donation rate and the increasing demand. In this context, this project aims to contribute to the scientific development by initiating the development of a new blood substitute. The main objectives of the project are to extract and purify bovine hemoglobin, to polymerize this hemoglobin (with glutaraldehyde, a succinimidic derivate of polyethylene glycol and phenylethydildiepoxide) and to structure the resulting polymer (with polylatic acid and polyethylene glycol).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KNIRSCH, MARCOS C.; DELL'ANNO, FILIPPO; CHICOMA, DENNIS; STEPHANO, MARCO ANTONIO; BOU-CHACRA, NADIA A.; PALOMBO, DOMENICO; CONVERTI, ATTILIO; POLAKIEWICZ, BRONISLAW. Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin. Bioprocess and Biosystems Engineering, v. 38, n. 11, p. 2263-2269, NOV 2015. (11/21933-0)

KNIRSCH, MARCOS CAMARGO; DELL'ANNO, FILIPPO; SALERNO, MARCO; LAROSA, CLAUDIO; POLAKIEWICZ, BRONISLAW; EGGENHOFFNER, ROBERTO; CONVERTI, ATTILIO. Combined characterization of bovine polyhemoglobin microcapsules by UV-Vis absorption spectroscopy and cyclic voltammetry. Bioprocess and Biosystems Engineering, v. 40, n. 3, p. 431-438, MAR 2017. (11/21933-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

KNIRSCH, Marcos Camargo. Synthesis and characterization of hemoglobin based oxygen carriers for therapeutic application. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.