Chemical study of Xanthomonas axonopodis pv. citri and its influence on chemical profile of Citrus sinensis

• Grant number: 11/22975-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: December 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Maria Fátima das Graças Fernandes da Silva
• Grantee: Edenilson dos Santos Niculau
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The citrus canker, caused by the bacterium X. axonopodis pv. citri, is an incurable disease that causes serious problems for the world citriculture, especially the orange plantations. The only way to eliminate the disease, since there is no adequate chemical control is the eradication of infected plants and plants suspicion. A further step to reduce the amount of inoculum of bacteria has been used by spraying copper-based. While sequencing the genome of X. axonopodis pv. citri has been completed, there is still no satisfactory control measures that may be recommended for this pathosystem. An alternative to growth inhibition, which is the mode of action of most traditional antibiotics, may be the attenuation of virulence factor production. Thus, an analysis of the metabolic events underlying bacterial cell-cell communication of signaling molecules may contribute to understanding of the virulence factor and consequently control the disease. In addition, there is a need in the search for new methods quick and easy for the identification and diagnostic of bacteria. Thus, the aims this project is to make the chemical study of X. axonopodis pv. citri in order to understand the chemical aspects involved in the development of bacteria, looking for possible signaling molecules of the virulence factor and make the study of production of volatiles in orange pear, Citrus sinensis (L.) Osbeck, with symptoms of citrus canker using Solid Phase Microextration (SPME) and dynamic headspace associated with GC-MS.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICULAU, EDENILSON DOS SANTOS; FERREIRA, DOUGLAS; RODRIGUES-FILHO, EDSON; BEHLAU, FRANKLIN; MAGNANI, RODRIGO FACCHINI; TOFFANO, LEONARDO; PRIETO, EVANDRO LUIS; FERNANDES, JOAO BATISTA; DA SILVA, MARIA FATIMA DAS GRACAS FERNANDES. MALDI-TOF Mass Spectrometry for the Diagnosis of Citrus Canker Caused by Xanthomonas citri subsp. citri. Molecules, v. 27, n. 24, p. 14-pg., 2022-12-01. (08/57859-5, 11/22975-8)

NICULAU, EDENILSON DOS SANTOS; RIBEIRO, LEANDRO DO PRADO; ANSANTE, THIAGO FELIPE; FERNANDES, JOAO BATISTA; FORIM, MOACIR ROSSI; VIEIRA, PAULO CEZAR; VENDRAMIM, JOSE DJAIR; DAS GRACAS FERNANDES DA SILVA, MARIA FATIMA. Isolation of Chavibetol and Methyleugenol from Essential Oil of Pimenta pseudocaryophyllus by High Performance Liquid Chromatography. Molecules, v. 23, n. 11, NOV 2018. (08/57859-5, 11/22975-8)