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EFFECT OF DIFFERENT DOSES AND APPLICATION INTERVALS OF LOW-LEVEL LASER THERAPY (905 nm) IN CYTOCHROME C-OXIDASE ACTIVITY IN DIFFERENT TYPES OF MUSCLE FIBERS OF SKELETAL MUSCLE IN RATS.

Grant number: 11/21145-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2012
Effective date (End): March 31, 2013
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Ernesto Cesar Pinto Leal Junior
Grantee:Victoria Nemeth
Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

Across the years modulation of cytochrome c-oxidase has been pointed as key-mechanism in interactionbetween LASER radiation with different biological tissues. More recently, a study demonstrated thatcytochrome c-oxidase activity is increased by phototherapy in different types of skeletal muscle fibers ofrats. Recent studies of our research group with phototherapy have shown positive results delaying skeletalmuscle fatigue and improving the status of biochemical markers related to skeletal muscle recovery whenthese therapies were applied before exercise. We think that cytochrome c-oxidase activity can play a key rolein low-level laser therapy (LLLT) effects in delaying skeletal muscle fatigue. With these perspectives inmind, this research project aims to evaluate effects of different LLLT doses in cytochrome c-oxidase activityin different types of skeletal muscle fibers, as well as to determine time interval that occurs peak of activityof cytochrome c-oxidase in different types of skeletal muscle fibers. For such, we will perform an animalexperiment. In this experiment tibialis anterior muscle of rats will be irradiated with different LLLT doses(1, 3 and 10 J), 905 nm wavelength, with 50 mW power output. After irradiation muscles will be removed atdifferent experimental times (5, 10, 30 minutes, 1, 2, 12 and 24 hours) and stored for further analysis.Analysis of cytochrome c-oxidase activity in different types of muscular fiber will be performed throughimmunohistochemical analysis, analysis of histological images, and Western Blotting.