Evaluation of adhesive/dentin interface, 2D and 3D imaging of marginal adaptation of Class I composite restorations and their bond-strength.

• Grant number: 11/22093-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2012
• End date: March 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Marcelo Giannini
• Grantee: Patricia Makishi
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated scholarship(s): 12/18583-0 - Evaluation of adhesive-dentin interface, 2D and 3D imaging of marginal adaptation of Class I composite restorations., BE.EP.PD

Abstract

Objectives: To evaluate the bond-strength of different restorative materials to superficial-dentin and their marginal adaptation in class I cavity. Methods: Round-shaped class I cavities (3 mm diameter x 1.5 mm depth) will be prepared in the oclusal surface of fresh extracted human third-molars, with margins located in superficial dentin. Four adhesive systems will be used and evaluated in this study; one two-step total-etch adhesive system (Adper Single Bond 2), one two-step self-etch adhesive system (Clearfil SE Bond) and two one-step adhesives (G-Bond, Clearfil S3), restored a with flowable resin (Clearfil Majesty LV) or a low-shrinkage composite with its proper adhesive (Filtek P-90, Silorane Adhesive System). The specimens will be immersed in water (24 h, 37°C) or thermal stress cycling (10000 cycles, 5°C and 55°C). Ammoniacal silver nitrate solution will be used to evaluate the marginal sealing ability and 3D scans (4 mm x 4 mm x 2.6 mm) of each restoration will be obtained using Optical Coherence Tomography (OCT). 2D images will be obtained from the 3D scans (n=15/group) and their marginal adaptation will be analyzed. Area fraction of pixels with high brightness values at the interfacial zone will be calculated using a digital image analysis software. The specimens will be sectioned and the interface will be visualized by SEM. Microtensile test will be used to measure their bond-strength in both storage periods. Results: The results of bond-strength test and marginal adaptation will be submitted to two-way ANOVA and Tukey test for statistical analysis.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MAKISHI, PATRICIA; THITTHAWEERAT, SUPPASON; SADR, ALIREZA; SHIMADA, YASUSHI; MARTINS, ADRIANO LUIS; TAGAMI, JUNJI; GIANNINI, MARCELO. Assessment of current adhesives in class I cavity: Nondestructive imaging using optical coherence tomography and microtensile bond strength. Dental Materials, v. 31, n. 9, p. E190-E200, SEP 2015. (12/18583-0, 11/22093-5)