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Evaluation of adhesive/dentin interface, 2D and 3D imaging of marginal adaptation of Class I composite restorations and their bond-strength

Grant number: 11/22093-5
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): April 01, 2012
Effective date (End): March 31, 2015
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Marcelo Giannini
Grantee:Patricia Makishi
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):12/18583-0 - Evaluation of adhesive-dentin interface, 2D and 3D imaging of marginal adaptation of class I composite restorations, BE.EP.PD


Objectives: To evaluate the bond-strength of different restorative materials to superficial-dentin and their marginal adaptation in class I cavity. Methods: Round-shaped class I cavities (3 mm diameter x 1.5 mm depth) will be prepared in the oclusal surface of fresh extracted human third-molars, with margins located in superficial dentin. Four adhesive systems will be used and evaluated in this study; one two-step total-etch adhesive system (Adper Single Bond 2), one two-step self-etch adhesive system (Clearfil SE Bond) and two one-step adhesives (G-Bond, Clearfil S3), restored a with flowable resin (Clearfil Majesty LV) or a low-shrinkage composite with its proper adhesive (Filtek P-90, Silorane Adhesive System). The specimens will be immersed in water (24 h, 37°C) or thermal stress cycling (10000 cycles, 5°C and 55°C). Ammoniacal silver nitrate solution will be used to evaluate the marginal sealing ability and 3D scans (4 mm x 4 mm x 2.6 mm) of each restoration will be obtained using Optical Coherence Tomography (OCT). 2D images will be obtained from the 3D scans (n=15/group) and their marginal adaptation will be analyzed. Area fraction of pixels with high brightness values at the interfacial zone will be calculated using a digital image analysis software. The specimens will be sectioned and the interface will be visualized by SEM. Microtensile test will be used to measure their bond-strength in both storage periods. Results: The results of bond-strength test and marginal adaptation will be submitted to two-way ANOVA and Tukey test for statistical analysis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAKISHI, PATRICIA; THITTHAWEERAT, SUPPASON; SADR, ALIREZA; SHIMADA, YASUSHI; MARTINS, ADRIANO LUIS; TAGAMI, JUNJI; GIANNINI, MARCELO. Assessment of current adhesives in class I cavity: Nondestructive imaging using optical coherence tomography and microtensile bond strength. Dental Materials, v. 31, n. 9, p. E190-E200, . (12/18583-0, 11/22093-5)

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