Study of the physical and functional interactions between eIF5A and the translation machinery using Saccharomyces cerevisiae as a model organismo

• Grant number: 12/02305-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sandro Roberto Valentini
• Grantee: Paulo Eduardo Gonçalves Boldrin
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 10/50044-6 - Study of the role of elF5A in translation elongation, AP.TEM
• Associated scholarship(s): 13/25261-1 - Analysis of interaction between the translation factor eIF5A and components of the Ribosome quality control (RQC) complex, BE.EP.DD

Abstract

Recent advances in eIF5A study has shown a significant impact on its function in the process of protein synthesis, specifically, the elongation step of translation. Results from our group using different conditional mutants of eIF5A in Saccharomyces cerevisiae revealed a functional relationship between eIF5A and eEF2. However, the mechanisms involved in the function of the translation elongation in elF5A remain unclear. In order to better correlate the role of eIF5A in the elongation process of translation, this work plan proposes the search for functional correlations between eIF5A and translation elongation factors eEF1A, eEF2 and eEF3 using genetic tests with mutant eIF5AQ22H/L93F and testing for physical interaction by two-hybrid and co-purification followed by polysomal profile. Thus, this request for direct doctorate fits the goal of a thematic project. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BOLDRIN, Paulo Eduardo Gonçalves. Investigation of the interactions between eIF5A and translational machinery and the Ribosome Quality Control complex, using Saccharomyces cerevisiae as model organism.. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.