Study of the impact of the role of eIF5A in the cell proteomic profile using Saccharomyces cerevisiae as a model

• Grant number: 16/16970-7
• Support type: Regular Research Grants
• Duration: June 01, 2017 - May 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal researcher: Cleslei Fernando Zanelli
• Grantee: Cleslei Fernando Zanelli
• Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The translation factor 5A (eIF5A) is highly conserved in Archaea and eukaryotes and is essential for cell viability. This is the only protein known to contain the amino acid residue hypusine, essential for eIF5A function, generated by a post-translational modification. The functional homolog of eIF5A in bacteria is the EF- P factor. The crystal structure of EF-P bound to 70S ribosome shows that this factor is located between the P and E sites and the modification loop near the peptidyl transferase center of the ribosome. Using data from tRNA and rRNA hydroxyl radical cleavage it was modeled the eIF5A location on 80S similarly as reported for EF-P structure. These structural data suggest an EF-P/eIF5A function in peptide bond formation if the E site is not occupied. Besides eIF5A and EF- P have already been associated with the translation initiation, more recent studies have established a role specifically on the elongation of consecutive proline-containing sequences (poly- P), which are able to induce a ribosome stalling due to low reactivity of proline for peptide bond formation. However, different proteomic cellular profile studies concluded that not only polypeptides containing poly-P have its translation dependent on EF- P and the absence of this factor does not affect all poly-P polipeptides. An analysis integrating data from different studies led to the conclusion that residues immediately before and immediately after consecutive proline residues inducing ribossmoal stalling and the translation initiation rate and poly-P sequence position also affect the dependence of EF- P for protein translation. Still, there are few studies with proteomic profile in the absence of eIF5A function and the proteins which syntheses are dependent on eIF5A are not well known. Thus, the present study aims to search for the proteins which syntheses are dependent on eIF5A by proteomic profile comparison between wild-type strains and eIF5A mutants in Saccharomyces cerevisiae. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANNOCHIO-RUSSO, HELENA; BUENO, PAULA CAROLINA P.; BAUERMEISTER, ANELIZE; DE ALMEIDA, RAFAEL FELIPE; DORRESTEIN, PIETER C.; CAVALHEIRO, ALBERTO JOSE; BOLZANI, VANDERLAN S. Can Statistical Evaluation Tools for Chromatographic Method Development Assist in the Natural Products Workflow? A Case Study on Selected Species of the Plant Family Malpighiaceae. Journal of Natural Products, v. 83, n. 11, p. 3239-3249, NOV 25 2020. Web of Science Citations: 0.

SILVA, SUELEN FERNANDES; KLIPPEL, ANGELICA HOLLUNDER; RAMOS, PRISCILA ZONZINI; SANTIAGO, ANDREE DA SILVA; VALENTINI, SANDRO ROBERTO; BENGTSON, MARIO HENRIQUE; MASSIRER, KATLIN BRAUER; BILSLAND, ELIZABETH; COUNAGO, RAFAEL MIGUEZ; ZANELLI, CLESLEI FERNANDO. Structural features and development of an assay platform of the parasite target deoxyhypusine synthase of Brugia malayi and Leishmania major. PLoS Neglected Tropical Diseases, v. 14, n. 10 OCT 2020. Web of Science Citations: 1.

NUNES, WILHAN DONIZETE GONCALVES; RUSSO, HELENA MANNOCHIO; BOLZANI, VANDERLAN DA SILVA; CAIRES, FLAVIO JUNIOR. Thermal characterization and compounds identification of commercialStevia rebaudianaBertoni sweeteners and thermal degradation products at high temperatures by TG-DSC, IR and LC-MS/MS. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, AUG 2020. Web of Science Citations: 0.

SABINO FERRARI, ANNA BEATRIZ; DE OLIVEIRA, GEORGE AZEVEDO; RUSSO, HELENA MANNOCHIO; BERTOZO, LUIZA CARVALHO; BOLZANI, VANDERLAN DA SILVA; ZIED, DIEGO CUNHA; XIMENES, VALDECIR FARIAS; ZERAIK, MARIA LUIZA. PleurotusostreatusandAgaricus subrufescens: investigation of chemical composition and antioxidant properties of these mushrooms cultivated with different handmade and commercial supplements. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 56, n. 1 JUL 2020. Web of Science Citations: 1.

RUSSO, HELENA MANNOCHIO; QUEIROZ, EMERSON FERREIRA; MARCOURT, LAURENCE; RUTZ, ADRIANO; ALLARD, PIERRE -MARIE; ALMEIDA, RAFAEL FELIPE DE; CARVALHO, NILTON MARQUES; WOLFENDER, JEAN-LUC; BOLZANI, VANDERLAN DA SILVA. Phytochemical analysis of the methanolic leaves extract of Niedenzuella multiglandulosa (Malpighiaceae), a plant species toxic to cattle in Brazil. PHYTOCHEMISTRY LETTERS, v. 37, p. 10-16, JUN 2020. Web of Science Citations: 0.

SCUPINARI, TAMIRES; RUSSO, HELENA MANNOCHIO; SABINO FERRARI, ANNA BEATRIZ; BOLZANI, VANDERLAN SILVA; DIAS, WALDIR PEREIRA; NUNES, ESTELA DE OLIVEIRA; HOFFMANN-CAMPO, CLARA BEATRIZ; ZERAIK, MARIA LUIZA. Crotalaria spectabilis as a source of pyrrolizidine alkaloids and phenolic compounds: HPLC-MS/MS dereplication and monocrotaline quantification of seed and leaf extracts. Phytochemical Analysis, v. 31, n. 6 MAY 2020. Web of Science Citations: 0.

BICALHO, KEYLLA U.; SANTONI, MARIANA M.; ARENDT, PHILIPP; ZANELLI, CLESLEI F.; FURLAN, MAYSA; GOOSSENS, ALAIN; POLLIER, JACOB. CYP712K4 Catalyzes the C-29 Oxidation of Friedelin in the Maytenus ilicifolia Quinone Methide Triterpenoid Biosynthesis Pathway. Plant and Cell Physiology, v. 60, n. 11, p. 2510-2522, NOV 2019. Web of Science Citations: 0.