Molecular and epidemiology analysis of the polymorphisms cyp1a1*2c and cyp2e1*6 in patients with colorectal Cancer

• Grant number: 12/00130-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2012
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Eny Maria Goloni Bertollo
• Grantee: Roliana Bravo Lelis
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Colorectal Cancer (CRC) is a malignant neoplasm that occurs in the large intestine (colon) and rectum, which is the predominant histologic type of adenocarcinoma. This type of cancer is the second most common in Western countries and has a high incidence in casuistry Brazilians, mainly in the southeast and south, according to epidemiological studies from INCA. Regarding the colorectal cancer etiology, some factors are already well established and it's possible to cite age over 60 years, first-degree relatives with intestine cancer, genetic syndromes such as familial adenomatous polyposis (FAP) and hereditary nonpolypoid Colorectal Cancer (HNPCC). Other factors such as chronic inflammatory bowel disease (ulcerative colitis or Crohn's disease), dietary factors, and life habits also raise the risk of CRC. Genetic polymorphisms may influence the metabolism of the carcinogen favoring the development of tumors in this anatomical region. Some studies reveal that there isn't an association between these cancers and polymorphisms in genes involved in CCR, such as CYP1A1 * 2A, CYP1A1 * 2C, CYP2E1 * 5B, CYP2E1 * 6, mEH exon 3 and mEH exon 4. This proposal has the objective of analyzing the frequency of six polymorphisms of these genes and investigating the relationship between them and the clinical and pathological characteristics of patients. The individuals will be submitted to the collection of peripheral blood genomic DNA extraction and molecular analysis. These data will be statistically analyzed and compared with the control group with no history of Cancer. The results of this project will contribute to understanding the involvement of xenobiotic metabolism and sporadic colorectal cancer in the elucidation of the mechanisms that lead to carcinogenesis.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PROENCA, M. A.; FERNANDES, G. M. M.; RUSSO, A.; LELIS, R. B.; NETINHO, J. G.; CUNRATH, G. S.; SILVA, A. E.; GOLONI-BERTOLLO, E. M.; PAVARINO, E. C.. A case-control study of CYP2E1 (PstI) and CYP1A1 (MspI) polymorphisms in colorectal cancer. Genetics and Molecular Research, v. 14, n. 4, p. 17856-17863, 2015. (12/00130-9, 12/02473-0, 11/23969-1)