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PATHOGENICITY OF "LINS" STRAIN OF Trypanosoma vivax IN NATURALLY AND EXPERIMENTALLY INFECTED CATTLE

Grant number: 11/15945-5
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Fabiano Antonio Cadioli
Grantee:Otávio Luiz Fidelis Júnior
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The aim of this project is detect hematological, biochemical, sorological and histopathological changes of naturally and experimentally infected Girolando cattle with Trypanosoma vivax. Eight adult female Girolando bovine will be used. Three naturally infected (N1 to N3); three experimentally infected (E1 to E3) with Tripanossoma vivax and two as control group (T1 and T2). The naturally infected animals were obtained during an outbreak in Lins (SP). Animals E1 to E3 will be inoculate IV with 2.0 x 107 T. vivax trypomastigotes. Blood samples will be obtain at each three days for hematological, parasitological and biochemical determinations. Serum samples will stored at -70°C until use. All samples will tested by IFAT and ELISA for anti-T. vivax antibodies. Eletrophoresis in SDS-PAGE for identification and quantification of acute phase proteins will perform. Animals will be euthanatized at 120 DPI or when show clinical and/or hematological life treating complications. Fragments from different tissues will collect and process for histological analysis.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; MARQUES, LUIZ CARLOS; CADIOLI, FABIANO ANTONIO. Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax. Revista Brasileira de Parasitologia Veterinária, v. 25, n. 1, p. 69-81, JAN-MAR 2016. Web of Science Citations: 5.
CADIOLI, FABIANO ANTONIO; FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; DOS SANTOS, GIULIANA NASCIMENTO; ANDRE, MARCOS ROGERIO; GARCIA DE ALMEIDA CASTILHO, KAYO JOSE; MACHADO, ROSANGELA ZACARIAS. Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods. Veterinary Parasitology, v. 214, n. 1-2, p. 174-177, NOV 30 2015. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JÚNIOR, Otávio Luiz Fidelis. Patogenicidade do isolado "Lins" de Trypanosoma vivax em bovinos natural e experimentalmente infectados. 2014. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.