LAPAROSCOPIC FOLLICULAR ASPIRATION AND IN VITRO MATURATION OF OOCYTES FROM PACAS (Cunniculus paca - Linnaeus, 1766) KEPT IN CAPTIVITY

• Grant number: 12/00724-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2012
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Wilter Ricardo Russiano Vicente
• Grantee: Felipe Farias Pereira da Câmara Barros
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Man's search for alternative sources of proteins for their nutrition enables the development of scientific research related to biotechnology of reproduction, where these are no longer carried out only for the conservation of the species, but also as a way to improve zoo technical production. With the scarcity of data in the literature, doubts arise about the possibility of obtaining viable oocytes from pacas to in vitro maturation. Therefore, the present study aims to establish a viable technique of follicular aspiration in pacas, evaluating the surgical procedure and reproductive aspects. For this, eight adult females will be submitted to four treatments each one, to collect oocytes through follicular aspiration by laparoscopy, in which three of these treatments received hormonal stimuli with eCG and FSH and one treatment will be realized without stimuli. The recovered oocytes in each treatment will be classified qualitatively and then submitted to in vitro maturation. The oocytes will be analyzed according to the stage of maturation. The collected data were expressed as mean ± standard deviation and subjected to analysis of variance (ANOVA) and Tukey's test (P<0.05) using the statistical program GraphPad Prism 4.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

USCATEGUI, RICARDO A. R.; BARROS, FELIPE F. P. C.; ALMEIDA, VIVIAN T.; KAWANAMI, ALINE E.; FELICIANO, MARCUS A. R.; VICENTE, WILTER R. R.. Evaluation of chemical restraint, isoflurane anesthesia and methadone or tramadol as preventive analgesia in spotted pacas (Cuniculus paca) subjected to laparoscopy. VETERINARY ANAESTHESIA AND ANALGESIA, v. 48, n. 1, p. 82-91, JAN 2021. (12/00724-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARROS, Felipe Farias Pereira da Câmara. Laparoscopic ovum pick-up and in vitro maturation in spotted pacas (Cuniculus paca - linnaeus, 1766) kept in captivity and subjected to ovarian superestimulation protocols. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.