Laparoscopic ovum pick-up and in vitro maturation in spotted pacas (Cuniculus paca - linnaeus, 1766) kept in captivity and subjected to ovarian superestimulation protocols

Author(s)

Felipe Farias Pereira da Câmara Barros

• Document type: Doctoral Thesis
• Press: Jaboticabal. 2016-03-14.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2016-02-17
• Advisor: Wilter Ricardo Russiano Vicente; Marcia Rita Fernandes Machado

Abstract

In recent years, with food shortages, the being human has been seeking possible alternative sources of protein for nutrition. Breeding of spotted pacas in captivity would be one of those alternatives, if exploited properly. Facts as that favor the development of scientific research related to biotechnology of reproduction, where these are no longer held solely because of a concern for conservation of this species, but also as a way to improve their zootechnical production. Due to lack of data in the literature doubts arise whether it would be possible to obtain viable oocytes from spotted pacas for in vitro maturation. Thus, the aim of this study was to establish viable technique of ovum pick-up in spotted pacas, testing effects of hormonal protocols on recovery rate of oocyte and IVM, and to evaluate the surgical and reproductive aspects. For this eight adult females were subjected to four treatments each, to collect oocytes by laparocoic ovum pick-up, which in three of these treatments were used hormonal stimulation with eCG and FSH. Retrieved oocytes in each treatment were classified qualitatively and then submitted to IVM. Subsequently, they were evaluated for nuclear maturation. After all analyzes, we conclude that the gonadotropin treatments were not efficient to hyperstimulate the treated groups or increased the oocyte recovery efficiency. Thus, further studies are needed, especially for new protocols, such as higher doses or frequency of administration as well as accuracy in the reproductive cycle times of treatments. (AU)

• FAPESP's process: 12/00724-6 - LAPAROSCOPIC FOLLICULAR ASPIRATION AND IN VITRO MATURATION OF OOCYTES FROM PACAS (Cunniculus paca - Linnaeus, 1766) KEPT IN CAPTIVITY
• Grantee: Felipe Farias Pereira da Câmara Barros
• Support Opportunities: Scholarships in Brazil - Doctorate