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In situ probe for real time viability and biomass density monitoring during ethanol fermentation aiming productivity improvements

Grant number: 12/01126-5
Support Opportunities:Scholarships abroad - Research
Start date: June 04, 2012
End date: March 03, 2013
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Valdinei Luís Belini
Grantee:Valdinei Luís Belini
Host Investigator: Hajo Suhr
Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Institution abroad: Hochschule Mannheim University of Applied Sciences, Germany  

Abstract

Traditionally the viability and yeasts density estimation have been carried out manually (hemacytometer). Although it is a relatively inexpensive and well known reagent-based off-line technique, it is error prone and has its inherent time-consuming issue in acquiring, preparing and analyzing the samples as main drawbacks. Under the hypothesis that information from consistent on-line and real time measurements may represent true yeasts fermentative conditions, the present research has the goals of proposing, implementing and evaluating the effectiveness of a probe based on the In Situ Microscopy (ISM) technique to monitor viability and yeast density directly from fermentation tanks during the entire fermentation process. The images obtained by this probe, neither outside sampling nor reagents, will be externally processed and analyzed by co-occurrence matrices and artificial neural network to automatically estimate the parameters of interest. It is intended, with this in situ technique, to be capable of detecting more accurately the optimal moment in which the alcoholic fermentation process has reached its maximum performance and must, therefore, be interrupted, aiming productivity improvements. The performance of the system will be demonstrated with statistically significant results from industrial fermentation operations. This proposal includes contextualization and justification of the problem under investigation, a synthesis of works related to the topic, predicted goals, expected results, plan of work with the execution schedule, materials and methodology, results analysis, and the justification for developing the research abroad. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BELINI, V. L.; CAURIN, G. A. P.; WIEDEMANN, P.; SUHR, H.. YEAST FERMENTATION OF SUGARCANE FOR ETHANOL PRODUCTION: CAN IT BE MONITORED BY USING IN SITU MICROSCOPY?. Brazilian Journal of Chemical Engineering, v. 34, n. 4, p. 949-959, . (12/01126-5)
BELINI, V. L.; CAURIN, G. A. P.; WIEDEMANN, P.; SUHR, H.. YEAST FERMENTATION OF SUGARCANE FOR ETHANOL PRODUCTION: CAN IT BE MONITORED BY USING IN SITU MICROSCOPY?. Brazilian Journal of Chemical Engineering, v. 34, n. 4, p. 11-pg., . (12/01126-5)
V. L. BELINI; G. A. P. CAURIN; P. WIEDEMANN; H. SUHR. Yeast fermentation of sugarcane for ethanol production: Can it be monitored by using in situ microscopy?. Brazilian Journal of Chemical Engineering, v. 34, n. 4, p. 949-959, . (12/01126-5)
BELINI, VALDINEI LUIS; WIEDEMANN, PHILIPP; SUHR, HAJO. In situ microscopy: A perspective for industrial bioethanol production monitoring. Journal of Microbiological Methods, v. 93, n. 3, p. 224-232, . (12/01126-5)