AbstractThe heat-labile toxins (LT) produced by enterotoxigenic Escherichia coli (ETEC) strains are, together with the cholera toxin (CT), some of the most studied mucosal adjuvants focusing both their academic interest and their potential use in applied areas of vaccine development. Although there are several reports describing the LT´s immunological activities, so far there are several gaps in the knowledge of these bacterial products that could permit more rational and appropriate use in vaccine formulations. The present project aims to evaluate the immunomodulatory properties of LT and some nontoxic derivatives (LT1-K63 and LT1-B) with regard to the specific serum antibody response elicited in parenterally vaccinated mice, particularly, regarding the glycosylation pattern of the immunoglobulin chains and how such patterns might affect some immunological features of these molecules. As a model antigen, a fragment of the type 2 dengue virus capsid protein (domain III of protein E) will be used. The present study shall contribute for the development of effective vaccine formulations against this pathogen.
News published in Agência FAPESP Newsletter about the scholarship: