RNA-seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle

• Grant number: 12/04087-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2012
• End date: September 30, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Isabel Kinney Ferreira de Miranda Santos
• Grantee: Sandra Regina Costa Maruyama
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 12/15464-0 - RNA-Seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle, BE.EP.PD

Abstract

Tick infestations by Rhipicephalus microplus, the cattle tick, cause enormous losses to animal health and consequently to animal production. Ticks induce immune response in their hosts, hence their immunobiological control is feasible. Due to variable efficacy of available anti-tick vaccine, several groups have tested new tick antigens. The research group in ticks of FMRP-USP is screening new candidate antigens, selected by transcriptomic analysis of ticks fed on resistant or susceptible hosts. So far, four recombinant salivary antigens have been tested as a multicomponent anti-tick vaccine in bovines. The R. microplus challenge displayed that tick infestation in vaccinated bovines was reduced significantly. The efficacy of vaccine (73.2%) was evaluated through antigen-specific antibody production and by reduction in biological parameters of infestation. A global perspective of the molecular process trigged during immunization and infestation in vaccinated bovines and control group will provide important information for understanding the mechanisms of protection involved in tick/host interface. Thus, the aim of this propose is analyzing the transcriptome of the leukocytes from peripheral blood of bovines vaccinated and of control group. The transcriptome was obtained by RNA-seq and the data will be analyzed by bioinformatic tools for differential gene expression identification, as well as obtaining gene signatures related to protective immunity conferred by the vaccine and also related to infestation.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANZIN, ALESSANDRA MARA; MARUYAMA, SANDRA REGINA; GARCIA, GUSTAVO ROCHA; OLIVEIRA, ROSANE PEREIRA; CHAVES RIBEIRO, JOSE MARCOS; BISHOP, RICHARD; MENDES MAIA, ANTONIO AUGUSTO; MORE, DANIELA DANTAS; FERREIRA, BEATRIZ ROSSETTI; DE MIRANDA SANTOS, ISABEL KINNEY FERREIRA. Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus. PARASITES & VECTORS, v. 10, JAN 31 2017. (12/04087-0)

MARUYAMA, SANDRA R.; GARCIA, GUSTAVO R.; TEIXEIRA, FELIPE R.; BRANDAO, LUCINDA G.; ANDERSON, JENNIFER M.; RIBEIRO, JOSE M. C.; VALENZUELA, JESUS G.; HORACKOVA, JANA; VERISSIMO, CECLIA J.; KATIKI, LUCIANA M.; et al. Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations. PARASITES & VECTORS, v. 10, APR 26 2017. (10/16464-8, 12/15464-0, 12/04087-0)

GARCIA, GUSTAVO ROCHA; MARUYAMA, SANDRA REGINA; NELSON, KRISTINA T.; CHAVES RIBEIRO, JOSE MARCOS; GARDINASSI, LUIZ GUSTAVO; MENDES MAIA, ANTONIO AUGUSTO; FERREIRA, BEATRIZ ROSSETTI; KOOYMAN, FRANS N. J.; DE MIRANDA SANTOS, ISABEL K. F.. Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host. PARASITES & VECTORS, v. 10, MAR 14 2017. (13/00382-0, 12/15464-0, 12/04087-0)

MARUYAMA, SANDRA R.; CARVALHO, BENILTON; GONZALEZ-PORTA, MAR; RUNG, JOHAN; BRAZMA, ALVIS; GARDINASSI, LUIZ GUSTAVO; FERREIRA, BEATRIZ R.; BANIN, TAMY M.; VERISSIMO, CECILIA J.; KATIKI, LUCIANA M.; et al. Blood transcriptome profile induced by an efficacious vaccine formulated with salivary antigens from cattle ticks. NPJ VACCINES, v. 4, n. 1, DEC 18 2019. (12/06708-2, 12/04087-0, 12/15464-0)

GARCIA, GUSTAVO R.; RIBEIRO, JOSE MARCOS CHAVES; MARUYAMA, SANDRA REGINA; GARDINASSI, LUIZ GUSTAVO; NELSON, KRISTINA; FERREIRA, BEATRIZ R.; ANDRADE, THALES GALDINO; FERREIRA DE MIRANDA SANTOS, ISABEL K.. A transcriptome and proteome of the tick Rhipicephalus microplus shaped by the genetic composition of its hosts and developmental stage. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 30 2020. (13/00382-0, 12/04087-0)