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Investigation of epigenetics mechanisms regulated by HIF1A and his target transcripts on medulloblastoma cell line after hypoxia exposition

Grant number: 12/06592-4
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): January 16, 2013
Effective date (End): May 15, 2013
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Elvis Terci Valera
Grantee:Gustavo Alencastro Veiga Cruzeiro
Supervisor abroad: Agda Karina Brodoloni Eterovic
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : University of Texas Health Science Center at Houston (UTHealth), United States  
Associated to the scholarship:11/04682-3 - HIF1A inhibition in meduloblastoma cell line under hypoxia enviroment, BP.MS

Abstract

Medulloblastoma is the most common brain cancer in childhood. The treatment available is surgery, chemiotherapy and radiotherapy. The use of more effective therapy, which grants a greater free disease survival and less later related effects it is very wanted. Thus, the search of new therapeutics targets became very important for childhood treatment. This targets can be related to cellular homeostatic regulations or epignetics mechanisms which confers more agressiveness and lethality tumors.Genes like HIF1A (hypoxia inducible factor), VEGF (vascular endothelial growth factor, CA9 (carbonic anhidrase 9) and SLCA2A1 (glucose transporter) are related with a poor prognosis in many types of human cancers. HIF1A, when activated, begin the transcription of various genes related to metastasis and resistance to treatment. However, the regulation of HIF1A it still unclear. In this project we aim to assess the presence of epigenetic mechanisms in HIF1A expression and his target transcripts (VEGF, CA9 and SLC2A1). We pretend to compare the methylation state and expression of these genes in distinct environments (hypoxia and normoxia). The methodology of this study includes HIF1A knockdown in cell line UW402 on hypoxia environment, pirosequencing and methylation array before/after gene inhibition. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VEIGA CRUZEIRO, GUSTAVO ALENCASTRO; DOS REIS, MARISTELLA BERGAMO; SILVEIRA, VANESSA SILVA; PEIXOTO LIRA, REGIA CAROLINE; CARLOTTI, CARLOS GILBERTO; NEDER, LUCIANO; OLIVEIRA, RICARDO SANTOS; YUNES, JOSE ANDRES; BRANDALISE, SILVIA REGINA; AGUIAR, SIMONE; ETEROVIC, AGDA KARINA; TONE, LUIZ GONZAGA; SCRIDELI, CARLOS ALBERTO; VALERA, ELVIS TERCI. HIF1A is Overexpressed in Medulloblastoma and its Inhibition Reduces Proliferation and Increases EPAS1 and ATG16L1 Methylation. CURRENT CANCER DRUG TARGETS, v. 18, n. 3, p. 287-294, 2018. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.