Clinical and laboratorial evaluation of an experimental solution based on Ricinus communis compared to sodium hypochlorite for denture cleansing

• Grant number: 12/08292-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2012
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Cláudia Helena Lovato da Silva
• Grantee: Maurício Malheiros Badaró
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of this study is to analyze by clinical and laboratorial evaluations the efficacy of an experimental solution based on Ricinus communis in comparison to sodium hypochlorite in cleaning total dentures. The clinical variables will be the effectiveness of biofilm removal from the inner surface of maxillary dentures, remission of chronic atrophic candidiasis and the patient's satisfaction degree. The laboratory variable will be the surface roughness of the acrylic resin after the use of the solutions. For clinical analysis, 40 upper denture users will be divided in groups: G1 - without chronic atrophic candidiasis and G2 - with chronic atrophic candidiasis, and they will be instructed to brush the prosthesis for 3 minutes, three times a day and to immerse them for 20 minutes in the solutions: S1 - distilled water (control); S2 - 0,25% Sodium Hypochlorite; S3 - 0,5% Sodium Hypochlorite and S4 - 10% Ricinus communis. The solutions will be used in a randomized sequence, in a crossed form for 07 days, with "washout" of 07 days. The biofilm quantification will be performed by the computerized method ImageTool 3.0. The candidiasis remission from G2 will be evaluated by assigning scores on palate images, before and after the use of the solutions. A questionnaire will be used to check the patient's satisfaction. For laboratory tests, the roughness will be measured (RI) before intervention in 40 specimens (90 x 30 x 4mm) of heat-polymerized acrylic resin. The specimens will be brushed three times a day for 3 minutes, immersed in the solutions for 20 minutes, rinsed and stored in artificial saliva (n = 40) during the intervals between the interventions. The trial period will be 21 days, with the roughness reading every 07 days. Then the specimens will be evaluated with scanning electron microscopy and this analysis will be qualitative. The solutions ability of biofilm removal will be evaluated by ANOVA (Two-way: solutions and groups) and Tukey's test. The candidiasis' remission will be analyzed by ANOVA (One-way) and Tukey's test. The analysis of the patients' satisfaction regarding the solutions will be performed in a descriptive way. The roughness data will be analyzed by ANOVA (Two-Way: Solutions and time) and Tukey's test. All analyzes will be conducted at 5% probability.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAURÍCIO MALHEIROS BADARÓ; MARCELA MOREIRA SALLES; VANESSA MARIA FAGUNDES LEITE; CAROLINA NORONHA FERRAZ DE ARRUDA; VIVIANE DE CÁSSIA OLIVEIRA; CÁSSIO DO NASCIMENTO; RAPHAEL FREITAS DE SOUZA; HELENA DE FREITAS DE OLIVEIRA PARANHOS; CLÁUDIA HELENA SILVA-LOVATO. Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser. Journal of Applied Oral Science, v. 25, n. 3, p. 324-334, 2017-06-00. (12/09834-9, 12/08292-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BADARÓ, Maurício Malheiros. Clinical and laboratorial evaluation of an experimental solution based on Ricinus communis compared to sodium hypochlorite for denture cleansing. 2013. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.